TY - JOUR
T1 - Identifying prognostic hub genes and key pathways in pediatric adrenocortical tumors through RNA sequencing and Co-expression analysis
AU - Veronez, Luciana Chain
AU - Xavier, Alcides Euzebio Tavares
AU - Nagano, Luiz Fernando
AU - Correa, Carolina Alves Pereira
AU - Borges, Kleiton Silva
AU - Santos, Paula
AU - Baroni, Mirella
AU - Silva Queiroz, Rosane de Paula
AU - Antonini, Sonir Roberto Rauber
AU - Yunes, José Andres
AU - Brandalise, Silvia Regina
AU - Molina, Carlos Augusto Fernandes
AU - Pinto, Emilia Modolo
AU - Valera, Elvis Terci
AU - Tone, Luiz Gonzaga
AU - Scrideli, Carlos Alberto
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/12/1
Y1 - 2024/12/1
N2 - Pediatric adrenocortical tumors (ACTs), rare conditions with uncertain prognoses, have high incidence in southern and southeastern Brazil. Pediatric ACTs are highly heterogeneous, so establishing prognostic markers for these tumors is challenging. We have conducted transcriptomic analysis on 14 pediatric ACT samples and compared cases with favorable and unfavorable clinical outcomes to identify prognostically significant genes. This comparison showed 1257 differentially expressed genes in favorable and unfavorable cases. Among these genes, 15 out of 60 hub genes were significantly associated with five-year event-free survival (EFS), and 10 had significant diagnostic value for predicting ACT outcomes in an independent microarray dataset of pediatric adrenocortical carcinomas (GSE76019). Overexpression of N4BP2, HSPB6, JUN, APBB1IP, STK17B, CSNK1D, and KDM3A was associated with poorer EFS, whereas lower expression of ISCU, PTPR, PRKAB2, CD48, PRF1, ITGAL, KLK15, and HIST1H3J was associated with worse outcomes. Collectively, these findings underscore the prognostic significance of these hub genes and suggest that they play a potential role in pediatric ACT progression and are useful predictors of clinical outcomes.
AB - Pediatric adrenocortical tumors (ACTs), rare conditions with uncertain prognoses, have high incidence in southern and southeastern Brazil. Pediatric ACTs are highly heterogeneous, so establishing prognostic markers for these tumors is challenging. We have conducted transcriptomic analysis on 14 pediatric ACT samples and compared cases with favorable and unfavorable clinical outcomes to identify prognostically significant genes. This comparison showed 1257 differentially expressed genes in favorable and unfavorable cases. Among these genes, 15 out of 60 hub genes were significantly associated with five-year event-free survival (EFS), and 10 had significant diagnostic value for predicting ACT outcomes in an independent microarray dataset of pediatric adrenocortical carcinomas (GSE76019). Overexpression of N4BP2, HSPB6, JUN, APBB1IP, STK17B, CSNK1D, and KDM3A was associated with poorer EFS, whereas lower expression of ISCU, PTPR, PRKAB2, CD48, PRF1, ITGAL, KLK15, and HIST1H3J was associated with worse outcomes. Collectively, these findings underscore the prognostic significance of these hub genes and suggest that they play a potential role in pediatric ACT progression and are useful predictors of clinical outcomes.
KW - hub genes
KW - pediatric adrenocortical tumor
KW - Prognosis
UR - http://www.scopus.com/inward/record.url?scp=85206697232&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2024.112383
DO - 10.1016/j.mce.2024.112383
M3 - Article
C2 - 39413985
AN - SCOPUS:85206697232
SN - 0303-7207
VL - 594
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
M1 - 112383
ER -