TY - JOUR
T1 - Identification of a second major site for CD46 binding in the hemagglutinin protein from a laboratory strain of measles virus (MV)
T2 - Potential consequences for wild-type MV infection
AU - Massé, Nicolas
AU - Barrett, Thomas
AU - Muller, Claude P.
AU - Wild, T. Fabian
AU - Buckland, Robin
PY - 2002/12
Y1 - 2002/12
N2 - Natural or wild-type (wt) measles virus (MV) infection in vivo which is restricted to humans and certain monkeys represents an enigma in terms of receptor usage. Although wt MV is known to use the protein SLAM (CD150) as a cell receptor, many human tissues, including respiratory epithelium in which the infection initiates, are SLAM negative. These tissues are CD46 positive, but wt MV strains, unlike vaccinal and laboratory MV strains, are not thought to use CD46 as a receptor. We have identified a novel CD46 binding site at residues S548 and F549, in the hemagglutinin (H) protein from a laboratory MV strain, which is also present in wt H proteins. Our results suggest that although wt MV interacts with SLAM with high affinity, it also possesses the capacity to interact with CD46 with low affinity.
AB - Natural or wild-type (wt) measles virus (MV) infection in vivo which is restricted to humans and certain monkeys represents an enigma in terms of receptor usage. Although wt MV is known to use the protein SLAM (CD150) as a cell receptor, many human tissues, including respiratory epithelium in which the infection initiates, are SLAM negative. These tissues are CD46 positive, but wt MV strains, unlike vaccinal and laboratory MV strains, are not thought to use CD46 as a receptor. We have identified a novel CD46 binding site at residues S548 and F549, in the hemagglutinin (H) protein from a laboratory MV strain, which is also present in wt H proteins. Our results suggest that although wt MV interacts with SLAM with high affinity, it also possesses the capacity to interact with CD46 with low affinity.
UR - http://www.scopus.com/inward/record.url?scp=0036892732&partnerID=8YFLogxK
U2 - 10.1128/JVI.76.24.13034-13038.2002
DO - 10.1128/JVI.76.24.13034-13038.2002
M3 - Article
C2 - 12438629
AN - SCOPUS:0036892732
SN - 0022-538X
VL - 76
SP - 13034
EP - 13038
JO - Journal of Virology
JF - Journal of Virology
IS - 24
ER -