Identification of a polygalacturonase (Cup s 2) as the major CCD-bearing allergen in Cupressus sempervirens pollen

Y. Shahali, J. P. Sutra, C. Hilger, K. Swiontek, I. Haddad, J. Vinh, L. Guilloux, D. Charpin, H. Sénéchal, P. Poncet*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

As IgE glyco-epitopes, also referred to as cross-reactive carbohydrate determinants (CCDs), can share significant structural homologies between different plants, they are prone to extensive cross-reactivity among allergen pollen extracts. Here, cypress pollen allergens, especially a polygalacturonase (PG), were further characterized using double one-dimensional electrophoresis (D1-DE). The presence of specific IgE directed against CCDs was investigated by bromelain IgE inhibition and concanavalin A binding assays using sera of cypress pollen-sensitized patients. Our results showed that IgE reactivity to CCDs in Cupressus sempervirens pollen extracts is mainly related to bromelain-type epitopes of a newly identified cypress PG. This glycoprotein has been further characterized through an immunoproteomic approach and officially indexed as Cup s 2 by the WHO/IUIS allergen nomenclature. Cup s 2 could thus be associated with the increased prevalence of IgE reactivity to cypress pollen extracts because of CCD interference.

Original languageEnglish
Pages (from-to)1806-1810
Number of pages5
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume72
Issue number11
DOIs
Publication statusPublished - 2017

Keywords

  • Cupressus sempervirens
  • bromelain
  • cross-reactive carbohydrate determinant
  • cypress pollen allergens
  • polygalacturonase

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