Hypoxic stress suppresses RNA polymerase III recruitment and tRNA gene transcription in cardiomyocytes

Isabelle Ernens, Sarah J. Goodfellow, Fiona Innes, Niall S. Kenneth, Louise E. Derblay, Robert J. White, Pamela H. Scott*

*Corresponding author for this work

    Research output: Contribution to journalArticleResearchpeer-review

    30 Citations (Scopus)


    RNA polymerase (pol) III transcription decreases when primary cultures of rat neonatal cardiomyocytes are exposed to low oxygen tension. Previous studies in fibroblasts have shown that the pol III-specific transcription factor IIIB (TFIIIB) is bound and regulated by the proto-oncogene product c-Myc, the mitogen-activated protein kinase ERK and the retinoblastoma tumour suppressor protein, RB. The principal function of TFIIIB is to recruit pol III to its cognate gene template, an activity that is known to be inhibited by RB and stimulated by ERK. We demonstrate by chromatin immunoprecipitation (ChIP) that c-Myc also stimulates pol III recruitment by TFIIIB. However, hypoxic conditions cause TFIIIB dissociation from c-Myc and ERK, at the same time as increasing its interaction with RB. Consistent with this, ChIP assays indicate that the occupancy of tRNA genes by pol III is significantly reduced, whereas promoter binding by TFIIIB is undiminished. The data suggest that hypoxia can inhibit pol III transcription by altering the interactions between TFIIIB and its regulators and thus compromising its ability to recruit the polymerase. These effects are independent of cell cycle changes.

    Original languageEnglish
    Pages (from-to)286-294
    Number of pages9
    JournalNucleic Acids Research
    Issue number1
    Publication statusPublished - Jan 2006


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