Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells

M. De Nicola; F. Liuzzi; C. Cerella; M. D'Alessio; A. Bergamaschi; A. A. Magrini; L. Ghibelli

doi:10.1196/annals.1299.076

Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells

M. De Nicola^*, F. Liuzzi, C. Cerella, M. D'Alessio, A. Bergamaschi, A. A. Magrini, L. Ghibelli

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

Tumor promonocytic U937 cells cultured under a low O₂/high CO₂ atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca²⁺ mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.

Original language	English
Pages (from-to)	426-429
Number of pages	4
Journal	Annals of the New York Academy of Sciences
Volume	1010
DOIs	https://doi.org/10.1196/annals.1299.076
Publication status	Published - 2003
Externally published	Yes

Keywords

Apoptosis
Glutathione
Hypoxia
U937

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10.1196/annals.1299.076

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title = "Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells",

abstract = "Tumor promonocytic U937 cells cultured under a low O2/high CO2 atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca2+ mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.",

keywords = "Apoptosis, Glutathione, Hypoxia, U937",

author = "{De Nicola}, M. and F. Liuzzi and C. Cerella and M. D'Alessio and A. Bergamaschi and Magrini, {A. A.} and L. Ghibelli",

year = "2003",

doi = "10.1196/annals.1299.076",

language = "English",

volume = "1010",

pages = "426--429",

journal = "Annals of the New York Academy of Sciences",

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TY - JOUR

T1 - Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells

AU - De Nicola, M.

AU - Liuzzi, F.

AU - Cerella, C.

AU - D'Alessio, M.

AU - Bergamaschi, A.

AU - Magrini, A. A.

AU - Ghibelli, L.

PY - 2003

Y1 - 2003

N2 - Tumor promonocytic U937 cells cultured under a low O2/high CO2 atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca2+ mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.

AB - Tumor promonocytic U937 cells cultured under a low O2/high CO2 atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca2+ mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.

KW - Apoptosis

KW - Glutathione

KW - Hypoxia

KW - U937

UR - http://www.scopus.com/inward/record.url?scp=1342347851&partnerID=8YFLogxK

U2 - 10.1196/annals.1299.076

DO - 10.1196/annals.1299.076

M3 - Article

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AN - SCOPUS:1342347851

SN - 0077-8923

VL - 1010

SP - 426

EP - 429

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells

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Keywords

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