Hyphenation of fast liquid chromatography with high-resolution mass spectrometry for quantitative proteomics analyses

Antoine Lesur, Sebastien Gallien, Bruno Domon*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

12 Citations (Scopus)

Abstract

Targeted analyses performed on high-resolution accurate mass spectrometers hyphenated to liquid chromatographs have opened new opportunities in quantitative proteomics. A new extension of the parallel reaction monitoring (PRM) technique uses the internal standards added to samples to dynamically control the data acquisition and maximize the analysis of the endogenous peptides. This account describes the hyphenation of ultra-HPLC with a high acquisition rate quadrupole-orbitrap instrument to properly sample the sharp elution profiles of UHPLC separations, possible with the IS-PRM technique.

Original languageEnglish
Pages (from-to)144-150
Number of pages7
JournalTrAC - Trends in Analytical Chemistry
Volume84
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • High-resolution mass spectrometry
  • Internal standard
  • Isotopically labeled peptides
  • Orbitrap mass spectrometry
  • PRM (Parallel Reaction Monitoring)
  • Target proteomics
  • Ultra UHPLC

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