Hyphenation of fast liquid chromatography with high-resolution mass spectrometry for quantitative proteomics analyses

Antoine Lesur, Sebastien Gallien, Bruno Domon*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    13 Citations (Scopus)

    Abstract

    Targeted analyses performed on high-resolution accurate mass spectrometers hyphenated to liquid chromatographs have opened new opportunities in quantitative proteomics. A new extension of the parallel reaction monitoring (PRM) technique uses the internal standards added to samples to dynamically control the data acquisition and maximize the analysis of the endogenous peptides. This account describes the hyphenation of ultra-HPLC with a high acquisition rate quadrupole-orbitrap instrument to properly sample the sharp elution profiles of UHPLC separations, possible with the IS-PRM technique.

    Original languageEnglish
    Pages (from-to)144-150
    Number of pages7
    JournalTrAC - Trends in Analytical Chemistry
    Volume84
    DOIs
    Publication statusPublished - 1 Nov 2016

    Keywords

    • High-resolution mass spectrometry
    • Internal standard
    • Isotopically labeled peptides
    • Orbitrap mass spectrometry
    • PRM (Parallel Reaction Monitoring)
    • Target proteomics
    • Ultra UHPLC

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