Targeted analyses performed on high-resolution accurate mass spectrometers hyphenated to liquid chromatographs have opened new opportunities in quantitative proteomics. A new extension of the parallel reaction monitoring (PRM) technique uses the internal standards added to samples to dynamically control the data acquisition and maximize the analysis of the endogenous peptides. This account describes the hyphenation of ultra-HPLC with a high acquisition rate quadrupole-orbitrap instrument to properly sample the sharp elution profiles of UHPLC separations, possible with the IS-PRM technique.
- High-resolution mass spectrometry
- Internal standard
- Isotopically labeled peptides
- Orbitrap mass spectrometry
- PRM (Parallel Reaction Monitoring)
- Target proteomics
- Ultra UHPLC