@inbook{e4e58f39f2314642b3a035b5f1e02108,
title = "How to distinguish between the activity of HDAC1-3 and HDAC6 with Western blot",
abstract = "Histone deacetylases (HDACs) catalyze the deacetylation of lysine residues in their target proteins. This biochemical modification can have profound effects on the functions of these proteins and a dysregulation of HDAC activity contributes to severe diseases, including neoplastic transformation. In the following chapter, we present a strategy that allows to distinguish between the inhibition of the class I HDACs HDAC1, 2, and 3 and of the class IIb HDAC HDAC6. This method is based on Western blot and relies on the detection of hyperacetylated substrates of class I or class IIb HDACs in lysates from cells that were treated with histone deacetylase inhibitors (HDACi).",
keywords = "Acetylation, HDAC6, HDACi, HDACs, Histone, Tubulin",
author = "Mandy Beyer and Nicole Kiweler and Siavosh Mahboobi and Kr{\"a}mer, {Oliver H.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6527-4_26",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "355--364",
booktitle = "Methods in Molecular Biology",
}