Abstract
HIV-1 Vpu induces downregulation of cell surface NTB-A to evade lysis of HIV-1 infected cells by NK cells. Here we show that Vpu affects the anterograde transport and the glycosylation pattern of NTB-A by a mechanism that is distinct from the Vpu induced downregulation of CD4 and tetherin. In the presence of Vpu, only the high mannose form of NTB-A was detectable, suggesting that Vpu prevented the formation of the mature form of NTB-A. This phenomenon is associated with the ability of Vpu to downregulate cell surface NTB-A by retention of NTB-A within the Golgi-compartment. Furthermore, the Vpu-mediated effect on NTB-A glycosylation is highly conserved among Vpu proteins derived from HIV-1 and SIV and corresponds to the level of downregulation of NTB-A. Together, these results suggest that the reduction of NTB-A from the cell surface is associated with the Vpu-mediated effect on the glycosylation pattern of newly synthesized NTB-A molecules.
Original language | English |
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Pages (from-to) | 190-203 |
Number of pages | 14 |
Journal | Virology |
Volume | 440 |
Issue number | 2 |
DOIs | |
Publication status | Published - 5 Jun 2013 |
Externally published | Yes |
Keywords
- HIV-1
- N-linked glycosylation
- NTB-A
- Pulse-chase
- SIV
- SLAMF6
- Tetherin
- Vpu