HIV-1 Vpu affects the anterograde transport and the glycosylation pattern of NTB-A

Sebastian Bolduan, Philipp Hubel, Tatjana Reif, Veronika Lodermeyer, Kristin Höhne, Joëlle V. Fritz, Daniel Sauter, Frank Kirchhoff, Oliver T. Fackler, Michael Schindler, Ulrich Schubert*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

HIV-1 Vpu induces downregulation of cell surface NTB-A to evade lysis of HIV-1 infected cells by NK cells. Here we show that Vpu affects the anterograde transport and the glycosylation pattern of NTB-A by a mechanism that is distinct from the Vpu induced downregulation of CD4 and tetherin. In the presence of Vpu, only the high mannose form of NTB-A was detectable, suggesting that Vpu prevented the formation of the mature form of NTB-A. This phenomenon is associated with the ability of Vpu to downregulate cell surface NTB-A by retention of NTB-A within the Golgi-compartment. Furthermore, the Vpu-mediated effect on NTB-A glycosylation is highly conserved among Vpu proteins derived from HIV-1 and SIV and corresponds to the level of downregulation of NTB-A. Together, these results suggest that the reduction of NTB-A from the cell surface is associated with the Vpu-mediated effect on the glycosylation pattern of newly synthesized NTB-A molecules.

Original languageEnglish
Pages (from-to)190-203
Number of pages14
JournalVirology
Volume440
Issue number2
DOIs
Publication statusPublished - 5 Jun 2013
Externally publishedYes

Keywords

  • HIV-1
  • N-linked glycosylation
  • NTB-A
  • Pulse-chase
  • SIV
  • SLAMF6
  • Tetherin
  • Vpu

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