High-throughput sequencing of murine immunoglobulin heavy chain repertoires using single side unique molecular identifiers on an Ion Torrent PGM

Keyphrases

• Personal Genome Machine 100%
• Single-side 100%
• Immunoglobulin Repertoire 100%
• Immunoglobulin Heavy Chain 100%
• Unique Molecular Identifiers 100%
• Ion Torrent Personal Genome Machine 100%
• Culling 25%
• Sequence Method 25%
• Bone Marrow Samples 25%
• Repertoire Sequencing 25%
• Processing Strategies 25%
• Nucleotide Insertion 25%
• Complementarity-determining Region 3 (CDR3) 25%
• Combining Errors 25%
• Sequencing Platforms 25%
• Immunogenetics 25%
• RNA Transcripts 25%
• Bulk Sequencing 25%
• Murine Bone Marrow 25%
• Barcoding 25%
• Sequencing Technology 25%
• Sequence Alignment 25%
• Deletion Detection 25%
• Frameshift 25%
• Ion Torrent 25%
• Error Correction 25%
• High-throughput Sequencing Data 25%
• Highly Accurate 25%
• Nucleotide Deletion 25%

Immunology and Microbiology

• Immunoglobulin Heavy Chain 100%
• High Throughput Sequencing 100%
• Intravenous Immunoglobulin 100%
• Indel Mutation 83%
• Sequence Alignment 16%
• Immunogenetics 16%

Biochemistry, Genetics and Molecular Biology

• Heavy Chain 100%
• Intravenous Immunoglobulin 100%
• High Throughput Sequencing 100%
• Indel 62%
• Sequence Alignment 12%
• Immunogenetics 12%
• Messenger RNA 12%