TY - JOUR
T1 - High-throughput microRNA profile in adult and pediatric primary glioblastomas
T2 - the role of miR-10b-5p and miR-630 in the tumor aggressiveness
AU - Junior, Luiz Guilherme Darrigo
AU - Baroni, Mirella
AU - Lira, Régia Caroline Peixoto
AU - Teixeira, Silvia
AU - Fedatto, Paola Fernanda
AU - Silveira, Vanessa Silva
AU - Suazo, Veridiana Kill
AU - Veronez, Luciana Chain
AU - Panepucci, Rodrigo Alexandre
AU - Antônio, David Santos Marco
AU - Yunes, José Andres
AU - Brandalise, Silvia Regina
AU - dos Santos Aguiar, Simone
AU - Neder, Luciano
AU - de Oliveira, Ricardo Santos
AU - Machado, Hélio Rubens
AU - Carlotti, Carlos Gilberto
AU - Tone, Luiz Gonzaga
AU - Valera, Elvis Terci
AU - Scrideli, Carlos Alberto
N1 - Publisher Copyright:
© 2020, Springer Nature B.V.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Glioblastoma (GBM) is the most common primary malignant neoplasm of the central nervous system and, despite the standard therapy; the patients’ prognoses remain dismal. The miRNA expression profiles have been associated with patient prognosis, suggesting that they may be helpful for tumor diagnosis and classification as well as predictive of tumor response to treatment. We described the microRNA expression profile of 29 primary GBM samples (9 pediatric GBMs) and 11 non-neoplastic white matter samples as controls (WM) by microarray analysis and we performed functional in vitro assays on these 2 most differentially expressed miRNAs. Hierarchical clustering analysis showed 3 distinct miRNA profiles, two of them in the GBM samples and a group consisting only of cerebral white matter. When adult and pediatric GBMs were compared to WM, 37 human miRNAs were found to be differentially expressed, with miR-10b-5p being the most overexpressed and miR-630 the most underexpressed. The overexpression of miR-630 was associated with reduced cell proliferation and invasion in the U87 GBM cell line, whereas the inhibition of miR-10b-5p reduced cell proliferation and colony formation in the U251 GBM cell line, suggesting that these miRNAs may act as tumor-suppressive and oncogenic miRNAs, respectively. The present study highlights the distinct epigenetic profiling of adult and pediatric GBMs and underscores the biological importance of mir-10b-5p and miR-630 for the pathobiology of these lethal tumors.
AB - Glioblastoma (GBM) is the most common primary malignant neoplasm of the central nervous system and, despite the standard therapy; the patients’ prognoses remain dismal. The miRNA expression profiles have been associated with patient prognosis, suggesting that they may be helpful for tumor diagnosis and classification as well as predictive of tumor response to treatment. We described the microRNA expression profile of 29 primary GBM samples (9 pediatric GBMs) and 11 non-neoplastic white matter samples as controls (WM) by microarray analysis and we performed functional in vitro assays on these 2 most differentially expressed miRNAs. Hierarchical clustering analysis showed 3 distinct miRNA profiles, two of them in the GBM samples and a group consisting only of cerebral white matter. When adult and pediatric GBMs were compared to WM, 37 human miRNAs were found to be differentially expressed, with miR-10b-5p being the most overexpressed and miR-630 the most underexpressed. The overexpression of miR-630 was associated with reduced cell proliferation and invasion in the U87 GBM cell line, whereas the inhibition of miR-10b-5p reduced cell proliferation and colony formation in the U251 GBM cell line, suggesting that these miRNAs may act as tumor-suppressive and oncogenic miRNAs, respectively. The present study highlights the distinct epigenetic profiling of adult and pediatric GBMs and underscores the biological importance of mir-10b-5p and miR-630 for the pathobiology of these lethal tumors.
KW - Glioblastoma
KW - miR-10b
KW - miR-630
KW - miRNA
UR - http://www.scopus.com/inward/record.url?scp=85090144560&partnerID=8YFLogxK
U2 - 10.1007/s11033-020-05754-3
DO - 10.1007/s11033-020-05754-3
M3 - Article
C2 - 32888124
AN - SCOPUS:85090144560
SN - 0301-4851
VL - 47
SP - 6949
EP - 6959
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 9
ER -