TY - JOUR
T1 - High-resolution proteomic profiling of spider venom
T2 - Expanding the toxin diversity of Phoneutria nigriventer venom
AU - Liberato, Tarcísio
AU - Troncone, Lanfranco Ranieri Paolo
AU - Yamashiro, Edson T.
AU - Serrano, Solange M.T.
AU - Zelanis, André
N1 - Publisher Copyright:
© 2016 Springer-Verlag.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.
AB - Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.
KW - Chitinase
KW - Phoneutria nigriventer
KW - Proteome
KW - Spider venom
UR - http://www.scopus.com/inward/record.url?scp=84958113867&partnerID=8YFLogxK
U2 - 10.1007/s00726-015-2151-6
DO - 10.1007/s00726-015-2151-6
M3 - Article
C2 - 26803659
AN - SCOPUS:84958113867
SN - 0939-4451
VL - 48
SP - 901
EP - 906
JO - Amino Acids
JF - Amino Acids
IS - 3
ER -