High correlation between the Roche COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1, v2.0 and the Abbott m2000 RealTime HIV-1 assays for quantification of viral load in HIV-1 B and non-B subtypes

J. C. Karasi, F. Dziezuk, L. Quennery, S. Förster, U. Reischl, G. Colucci, D. Schoener, C. Seguin-Devaux*, J. C. Schmit

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Abstract

Background: HIV-1 viral load assays are critical tools to monitor antiretroviral therapy efficacy in HIV-infected patients. Two assays based on real-time PCR are available, the Abbott Real-Time HIV-1 assay (Abbott assay) and the new Roche COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 test, v. 2.0 (TaqMan ® test v2.0). Objectives: We have compared the performance of the two assays in 546 clinical plasma specimens of group M strains from Luxembourg and Rwanda. Study design: Our analyses focused on subtype inclusivity and platforms accuracy for 328 low level viremia samples. Results: Strong agreement and linear correlation were observed between the two assays (R 2=0.95) over a wide dynamic range. Bland-Altman analysis showed a mean difference of 0.04log10 indicating minimal overall viral load quantification differences between both platforms. One subtype C was severely underquantified by TaqMan ® test v2.0 for which sequence analysis revealed multiple mismatches between the viral sequence and the primer/probe regions. A non significant lower quantification of the Abbott assay was shown for subtype A1 with a mean log10 difference of 0.24. For specimens under 200cp/mL, the overall agreement was 90% at the cut-off of 50cp/mL and 67% at assay's lower limit of detection of 20 and 40cp/mL. 309 samples were retested by the COBAS ® AMPLICOR ® HIV-1 MONITOR Test, v. 1.5 and a lack of agreement between the three assays around their lower limit of quantification was revealed. Conclusions: Both real-time tests were closely comparable in the quantification of viral load specimens of ten HIV-1 subtypes and recombinant forms.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalJournal of Clinical Virology
Volume52
Issue number3
DOIs
Publication statusPublished - Nov 2011

Keywords

  • HIV-1
  • Low level viremia
  • Real-time PCR
  • Subtype
  • Viral-load

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