Global analysis of cellular protein translation by pulsed SILAC

Björn Schwanhäusser, Manfred Gossen, Gunnar Dittmar, Matthias Selbach*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

245 Citations (Scopus)

Abstract

Current methods for system-wide gene expression analysis detect changes in mRNA abundance, but neglect regulation at the level of translation. Pulse labeling with stable isotopes has been used to measure protein turnover rates, but this does not directly provide information about translation rates. Here, we developed pulsed stable isotope labeling by amino acids in cell culture (pSI-LAC) with two heavy isotope labels to directly quantify protein translation on a proteome-wide scale. We applied the method to cellular iron homeostasis as a model system and demonstrate that it can confidently identify proteins that are translationally regulated by iron availability.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalProteomics
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Keywords

  • Iron homeostasis
  • SILAC
  • Translation regulation

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