Genomic organization and characterization of the promoter of the human ATP-binding cassette transporter-G1 (ABCG1) gene

Thomas Langmann, Mustafa Porsch-Özcürümez, Uwe Unkelbach, Jochen Klucken, Gerd Schmitz*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)

Abstract

The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and phospholipid transport. This transporter together with ABCA1 belongs to a group of sterol-sensitive ABC proteins which are induced by lipid loading or specific oxysterols. We report here the genomic structure of ABCG1 along with the 5' flanking sequence using library screening and BLAST search analysis. The ABCG1 gene spans more than 70 kb and contains 15 exons. The exon size is between 30 and 1081 bp and the introns range in size from 137 bp to more than 45 kb. All exon-intron boundaries display the canonical GT/AG sequences. Using promoter-luciferase reporter assays in the myeloid cell lines THP-1 and RAW246.7 and the hepatoma cell line HepG2 we could demonstrate the functionality of the ABCG1 promoter and the minimal sequence requirements for gene expression. The TATA-less proximal promoter contains multiple Sp1 binding sites and a consensus sequence for sterol regulatory element binding protein. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1494
Issue number1-2
DOIs
Publication statusPublished - 15 Nov 2000
Externally publishedYes

Keywords

  • ATP-binding cassette transporter G1
  • Cholesterol transport
  • Genomic organization
  • Promoter region
  • Sp1
  • Sterol regulatory element binding protein

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