Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1

Axel Chemla, Giuseppe Arena*, Gizem Onal, Jonas Walter, Clara Berenguer-Escuder, Dajana Grossmann, Anne Grünewald, Jens C. Schwamborn, Rejko Krüger*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).

Original languageEnglish
Article number103145
JournalStem Cell Research
Volume71
Early online date14 Jun 2023
DOIs
Publication statusPublished - Sept 2023

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