Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke; Zoé Hanss; Javier Jarazo; François Massart; Arkadiusz Rybicki; Elizabet Petkovski; Enrico Glaab; Ibrahim Boussaad; Vincenzo Bonifati; Jens Christian Schwamborn; Wim Mandemakers; Rejko Krüger

doi:10.1016/j.scr.2022.102815

Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke
, Zoé Hanss
, Javier Jarazo
, François Massart
, Arkadiusz Rybicki
, Elizabet Petkovski
, Enrico Glaab
, Ibrahim Boussaad
, Vincenzo Bonifati
, Jens Christian Schwamborn
, Wim Mandemakers
, Rejko Krüger^*

^*Corresponding author for this work

Transversal Translational Medicine

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.

Original language	English
Article number	102815
Journal	Stem Cell Research
Volume	62
Early online date	17 May 2022
DOIs	https://doi.org/10.1016/j.scr.2022.102815
Publication status	Published - Jul 2022

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10.1016/j.scr.2022.102815Licence: CC BY-NC-ND

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Mencke, P., Hanss, Z., Jarazo, J., Massart, F., Rybicki, A., Petkovski, E., Glaab, E., Boussaad, I., Bonifati, V., Christian Schwamborn, J., Mandemakers, W., & Krüger, R. (2022). Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1). Stem Cell Research, 62, Article 102815. https://doi.org/10.1016/j.scr.2022.102815

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title = "Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson{\textquoteleft}s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)",

abstract = "We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471\_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.",

author = "Pauline Mencke and Zo{\'e} Hanss and Javier Jarazo and Fran{\c c}ois Massart and Arkadiusz Rybicki and Elizabet Petkovski and Enrico Glaab and Ibrahim Boussaad and Vincenzo Bonifati and \{Christian Schwamborn\}, Jens and Wim Mandemakers and Rejko Kr{\"u}ger",

note = "Funding Information: The current work was supported by the Fonds National de Recherche (FNR) within the PEARL Excellence Programme [FNR/P13/6682797] to RK, and the MiRisk project [C17/BM/11676395], and by the Stichting ParkinsonFonds, The Netherlands [grant SPF-1870]. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = jul,

doi = "10.1016/j.scr.2022.102815",

language = "English",

volume = "62",

journal = "Stem Cell Research",

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publisher = "Elsevier B.V.",

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Mencke, P, Hanss, Z, Jarazo, J, Massart, F, Rybicki, A, Petkovski, E, Glaab, E, Boussaad, I, Bonifati, V, Christian Schwamborn, J, Mandemakers, W & Krüger, R 2022, 'Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)', Stem Cell Research, vol. 62, 102815. https://doi.org/10.1016/j.scr.2022.102815

TY - JOUR

T1 - Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

AU - Mencke, Pauline

AU - Hanss, Zoé

AU - Jarazo, Javier

AU - Massart, François

AU - Rybicki, Arkadiusz

AU - Petkovski, Elizabet

AU - Glaab, Enrico

AU - Boussaad, Ibrahim

AU - Bonifati, Vincenzo

AU - Christian Schwamborn, Jens

AU - Mandemakers, Wim

AU - Krüger, Rejko

N1 - Funding Information: The current work was supported by the Fonds National de Recherche (FNR) within the PEARL Excellence Programme [FNR/P13/6682797] to RK, and the MiRisk project [C17/BM/11676395], and by the Stichting ParkinsonFonds, The Netherlands [grant SPF-1870]. Publisher Copyright: © 2022 The Author(s)

PY - 2022/7

Y1 - 2022/7

N2 - We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.

AB - We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.

UR - https://www.scopus.com/pages/publications/85130629717

UR - https://pubmed.ncbi.nlm.nih.gov/35617842

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DO - 10.1016/j.scr.2022.102815

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C2 - 35617842

SN - 1873-5061

VL - 62

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102815

ER -

Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Abstract

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