Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)

Pauline Mencke, Zoé Hanss, Javier Jarazo, François Massart, Arkadiusz Rybicki, Elizabet Petkovski, Enrico Glaab, Ibrahim Boussaad, Vincenzo Bonifati, Jens Christian Schwamborn, Wim Mandemakers, Rejko Krüger*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes.

Original languageEnglish
Article number102815
JournalStem Cell Research
Volume62
Early online date17 May 2022
DOIs
Publication statusPublished - Jul 2022

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