Gas chromatography-tandem mass spectrometry analysis of 52 monohydroxylated metabolites of polycyclic aromatic hydrocarbons in hairs of rats after controlled exposure

Nathalie Grova*, Guillaume Salquèbre, Brice M.R. Appenzeller

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

A method based on gas chromatography-tandem mass spectrometry after derivatization with N-tert - butyldimethylsilyl-N-methyltrifluoroacetamide was developed for the analysis of monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in hair. The method focused on 52 target compounds corresponding to two- to six-ring monohydroxylated metabolites of polycyclic aromatic hydrocarbons (PAHs). The limits of quantification ranged from 0.2 to 50 pg mg-1. The method was then applied to the analysis of hair samples collected from rats exposed to 12 PAHs at 0.01, 0.1, and 1 mg kg-1, by intraperitoneal injection, for 28 days. The results of this study confirm that these metabolites can be incorporated in hair after intraperitoneal administration of the corresponding parent compound. Only 20 of the 52 metabolites were actually detected in hair samples and corresponded to nine parent PAHs. The mean concentrations ofOH-PAHs in rat hair samples exposed to PAHs at 1 mg kg-1 ranged from 0.6±0.2 pg mg-1 for 8-hydroxybenzo[b]fluoranthene to 6.7± 1.0 pg mg-1 for 1-hydroxypyrene. The results also demonstrated that hair pigmentation has no influence on the concentration of most OH-PAHs. This animal experiment confirmed the incorporation of PAH metabolites in hair and demonstrated that the method was sufficiently sensitive to detect low levels of exposure to PAHs. These results confirmed the usefulness of hair analysis in the biomonitoring of human exposure to PAHs.

Original languageEnglish
Pages (from-to)8897-8911
Number of pages15
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number27
DOIs
Publication statusPublished - Nov 2013

Keywords

  • Analysis
  • Biomonitoring
  • Gas chromatography-electron ionization tandem mass spectrometry
  • Hair
  • Monohydroxylated polycyclic aromatic hydrocarbons
  • Polycyclic aromatic hydrocarbons

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