Functional analysis of EID1, an F-box protein involved in phytochrome A-dependent light signal transduction

Katia Marrocco, Yongchun Zhou, Erik Bury, Monika Dieterle, Markus Funk, Pascal Genschik, Martina Krenz, Thorsten Stolpe, Thomas Kretsch*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

67 Citations (Scopus)

Abstract

Empfindlicher im Dunkelroten Licht 1 (EID1) is an F-box protein that functions as a negative regulator in phytochrome A (phyA)-specific light signalling. F-box proteins are components of SCF ubiquitin ligase complexes that target proteins for degradation in the proteasome. Here we present further characterization of EID1 at the expression level, and show that it regulates photomorphogenesis in seedlings, rosette leaf development and flowering. Data on transcript expression patterns indicate that EID1 is expressed during all stages of Arabidopsis development and exhibits no light response. Microscope studies demonstrate that EID1 is localized to the nucleus, where it can form speckles under continuous far-red light that resemble clastosomes. To characterize the composition and formation of SCFEID1 complexes further, we used two-hybrid and bridge assays in yeast and in planta. EID1 interacts specifically with several Arabidopsis Skp1-like (ASK) proteins and Cullin1 to form stable dimeric and trimeric complexes. Our results support a two-step association process in which the F-box protein binds first to the ASK adaptor, forming a unit which then associates with the catalytic core of the SCF complex. Finally, our data indicate that the EID1 target interaction domain is composed of two independent modules.

Original languageEnglish
Pages (from-to)423-438
Number of pages16
JournalPlant Journal
Volume45
Issue number3
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Arabidopsis
  • BiFC
  • EID1
  • Light signalling
  • Localization
  • SCF complex

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