Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: Correlation to MYCN amplification and effects of retinoic acid

K. David; A. Ehrhardt; M. W. Ollert; R. Erttmann; R. Bredehorst; C. W. Vogel

doi:10.1016/S0959-8049(97)00207-4

Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: Correlation to MYCN amplification and effects of retinoic acid

K. David, A. Ehrhardt, M. W. Ollert, R. Erttmann, R. Bredehorst, C. W. Vogel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non- amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.

Original language	English
Pages (from-to)	1937-1941
Number of pages	5
Journal	European Journal of Cancer
Volume	33
Issue number	12
DOIs	https://doi.org/10.1016/S0959-8049(97)00207-4
Publication status	Published - Oct 1997
Externally published	Yes

Keywords

MYCN
Natural antibodies
Neuroblastoma
Retinoid acid
Tumour antigen

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10.1016/S0959-8049(97)00207-4

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title = "Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: Correlation to MYCN amplification and effects of retinoic acid",

abstract = "Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non- amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.",

keywords = "MYCN, Natural antibodies, Neuroblastoma, Retinoid acid, Tumour antigen",

author = "K. David and A. Ehrhardt and Ollert, {M. W.} and R. Erttmann and R. Bredehorst and Vogel, {C. W.}",

note = "Funding Information: This work was supported in part by the Deutsche Krebshilfe e.V. and the F{\"o}rdergemeinschaft Kinderkrebszentrum Hamburg e.V.",

year = "1997",

month = oct,

doi = "10.1016/S0959-8049(97)00207-4",

language = "English",

volume = "33",

pages = "1937--1941",

journal = "European Journal of Cancer",

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T1 - Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM

T2 - Correlation to MYCN amplification and effects of retinoic acid

AU - David, K.

AU - Ehrhardt, A.

AU - Ollert, M. W.

AU - Erttmann, R.

AU - Bredehorst, R.

AU - Vogel, C. W.

N1 - Funding Information: This work was supported in part by the Deutsche Krebshilfe e.V. and the Fördergemeinschaft Kinderkrebszentrum Hamburg e.V.

PY - 1997/10

Y1 - 1997/10

N2 - Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non- amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.

AB - Human neuroblastoma cells contain a 260 kDa surface-associated antigen (NB-p260) that is recognised by natural cytotoxic IgM antibodies. In this study we demonstrate that NB-p260 is expressed in vivo in a neuroblastoma tumour specimen but not in normal human tissues of neuronal origin. Since MYCN amplification is a clinical marker of neuroblastoma disease progression, we analysed the expression of NB-p260 in human neuroblastoma cell lines with different MYCN amplification status. However, both amplified and non- amplified neuroblastoma cell lines exhibited comparable NB-p260 expression. Treatment of neuroblastoma cells with the differentiation-inducing agent retinoic acid (RA) also had no effect on the expression of NB-p260. Collectively, the data suggest that expression of NB-p260 on human neuroblastoma cells is independent of malignancy and differentiation status of neuroblastoma.

KW - MYCN

KW - Natural antibodies

KW - Neuroblastoma

KW - Retinoid acid

KW - Tumour antigen

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U2 - 10.1016/S0959-8049(97)00207-4

DO - 10.1016/S0959-8049(97)00207-4

M3 - Article

C2 - 9516828

AN - SCOPUS:0031427365

SN - 0959-8049

VL - 33

SP - 1937

EP - 1941

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 12

ER -

Expression of a 260 kDa neuroblastoma surface antigen, the target of cytotoxic natural human IgM: Correlation to MYCN amplification and effects of retinoic acid

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Keywords

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