Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila

Delphine Galiana-Arnoux, Catherine Dostert, Anette Schneemann, Jules A. Hoffmann, Jean Luc Imler*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

450 Citations (Scopus)

Abstract

The fruit fly Drosophila melanogaster is a model system for studying innate immunity, including antiviral host defense. Infection with drosophila C virus triggers a transcriptional response that is dependent in part on the Jak kinase Hopscotch. Here we show that successful infection and killing of drosophila with the insect nodavirus flock house virus was strictly dependent on expression of the viral protein B2, a potent inhibitor of processing of double-stranded RNA mediated by the essential RNA interference factor Dicer. Conversely, flies with a loss-of-function mutation in the gene encoding Dicer-2 (Dcr-2) showed enhanced susceptibility to infection by flock house virus, drosophila C virus and Sindbis virus, members of three different families of RNA viruses. These data demonstrate the importance of RNA interference for controlling virus replication in vivo and establish Dcr-2 as a host susceptibility locus for virus infections.

Original languageEnglish
Pages (from-to)590-597
Number of pages8
JournalNature Immunology
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila'. Together they form a unique fingerprint.

Cite this