Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila

Delphine Galiana-Arnoux; Catherine Dostert; Anette Schneemann; Jules A. Hoffmann; Jean Luc Imler

doi:10.1038/ni1335

Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila

Delphine Galiana-Arnoux, Catherine Dostert, Anette Schneemann, Jules A. Hoffmann, Jean Luc Imler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

461 Citations (Scopus)

Abstract

The fruit fly Drosophila melanogaster is a model system for studying innate immunity, including antiviral host defense. Infection with drosophila C virus triggers a transcriptional response that is dependent in part on the Jak kinase Hopscotch. Here we show that successful infection and killing of drosophila with the insect nodavirus flock house virus was strictly dependent on expression of the viral protein B2, a potent inhibitor of processing of double-stranded RNA mediated by the essential RNA interference factor Dicer. Conversely, flies with a loss-of-function mutation in the gene encoding Dicer-2 (Dcr-2) showed enhanced susceptibility to infection by flock house virus, drosophila C virus and Sindbis virus, members of three different families of RNA viruses. These data demonstrate the importance of RNA interference for controlling virus replication in vivo and establish Dcr-2 as a host susceptibility locus for virus infections.

Original language	English
Pages (from-to)	590-597
Number of pages	8
Journal	Nature Immunology
Volume	7
Issue number	6
DOIs	https://doi.org/10.1038/ni1335
Publication status	Published - Jun 2006
Externally published	Yes

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title = "Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila",

abstract = "The fruit fly Drosophila melanogaster is a model system for studying innate immunity, including antiviral host defense. Infection with drosophila C virus triggers a transcriptional response that is dependent in part on the Jak kinase Hopscotch. Here we show that successful infection and killing of drosophila with the insect nodavirus flock house virus was strictly dependent on expression of the viral protein B2, a potent inhibitor of processing of double-stranded RNA mediated by the essential RNA interference factor Dicer. Conversely, flies with a loss-of-function mutation in the gene encoding Dicer-2 (Dcr-2) showed enhanced susceptibility to infection by flock house virus, drosophila C virus and Sindbis virus, members of three different families of RNA viruses. These data demonstrate the importance of RNA interference for controlling virus replication in vivo and establish Dcr-2 as a host susceptibility locus for virus infections.",

author = "Delphine Galiana-Arnoux and Catherine Dostert and Anette Schneemann and Hoffmann, {Jules A.} and Imler, {Jean Luc}",

note = "Funding Information: We thank E. Santiago for technical assistance; S. Ozkan and R. Walther for help with transgenesis; D. Zachary for help with electron microscopy; R. Carthew for Dicer-2 mutant lines; J. Strauss for anti-SINV; J. McCauley for titered stock of SINV; and O. Voinnet for discussions and comments. Supported by Centre National de la Recherche Scientifique, Minist{\`e}re de la Technologie et de l{\textquoteright}Enseignement Sup{\'e}rieur (ACI Microbiologie to J.-L.I.), the National Institutes of Health (GM053491 to A.S.), a Centre National de la Recherche Scientifique postdoctoral fellowship (D.G.A.) and the Minist{\`e}re de la Recherche du Grand-Duch{\'e} du Luxembourg (C.D.).",

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doi = "10.1038/ni1335",

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AU - Galiana-Arnoux, Delphine

AU - Dostert, Catherine

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AU - Hoffmann, Jules A.

AU - Imler, Jean Luc

N1 - Funding Information: We thank E. Santiago for technical assistance; S. Ozkan and R. Walther for help with transgenesis; D. Zachary for help with electron microscopy; R. Carthew for Dicer-2 mutant lines; J. Strauss for anti-SINV; J. McCauley for titered stock of SINV; and O. Voinnet for discussions and comments. Supported by Centre National de la Recherche Scientifique, Ministère de la Technologie et de l’Enseignement Supérieur (ACI Microbiologie to J.-L.I.), the National Institutes of Health (GM053491 to A.S.), a Centre National de la Recherche Scientifique postdoctoral fellowship (D.G.A.) and the Ministère de la Recherche du Grand-Duché du Luxembourg (C.D.).

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Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila

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