Epidemiological study of p53 tumor suppressor gene mutations in patients from Luxembourg and the German Saar region with an advanced colorectal cancer using PCR-SSCP analysis

M. Pauly*, M. Schmitz, I. Kayser, P. Lagoda, Ö Türeci, A. Kerschen, J. Weber, G. Seitz, F. Hentges, M. Dicato

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Mutations in the p53 tumor suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoran to the carcinoma stage. We used the polymerase chain reaction (PCR) followed by single-strand conformation polymorphism (SSCP) analysis to screen for the presence of mutations in the p53 gene of patients from Luxembourg and the German Saar region with colorectal cancers at various developmental stages. While we detected no mutations in 16 colic polypi at an early to intermediate stage (adenoma), we revealed seven (13.7%) non-silent point mutations (transitions) in exons 5 to 9 of the p53 gene in 51 colorectal tumors at a late stage (carcinoma). In addition to confirming previous observations, these results show that PCR-SSCP analysis can provide both a sensitive and rapid method for the genetic determination of the histopathological stage of colorectal samples.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalBiomedicine and Pharmacotherapy
Volume52
Issue number5
DOIs
Publication statusPublished - Jun 1998
Externally publishedYes

Keywords

  • Colorectal cancer
  • Luxembourg-Saar region
  • P53 mutations

Fingerprint

Dive into the research topics of 'Epidemiological study of p53 tumor suppressor gene mutations in patients from Luxembourg and the German Saar region with an advanced colorectal cancer using PCR-SSCP analysis'. Together they form a unique fingerprint.

Cite this