Abstract
Mutations in the p53 tumor suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoran to the carcinoma stage. We used the polymerase chain reaction (PCR) followed by single-strand conformation polymorphism (SSCP) analysis to screen for the presence of mutations in the p53 gene of patients from Luxembourg and the German Saar region with colorectal cancers at various developmental stages. While we detected no mutations in 16 colic polypi at an early to intermediate stage (adenoma), we revealed seven (13.7%) non-silent point mutations (transitions) in exons 5 to 9 of the p53 gene in 51 colorectal tumors at a late stage (carcinoma). In addition to confirming previous observations, these results show that PCR-SSCP analysis can provide both a sensitive and rapid method for the genetic determination of the histopathological stage of colorectal samples.
Original language | English |
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Pages (from-to) | 220-228 |
Number of pages | 9 |
Journal | Biomedicine and Pharmacotherapy |
Volume | 52 |
Issue number | 5 |
DOIs | |
Publication status | Published - Jun 1998 |
Externally published | Yes |
Keywords
- Colorectal cancer
- Luxembourg-Saar region
- P53 mutations