Emissions from a modern log wood masonry heater and wood pellet boiler: Composition and biological impact on air-liquid interface exposed human lung cancer cells

Kanashova Tamara, Sippula Olli, Oeder Sebastian, Streibel Thorsten, Passig Johannes, Czech Hendryk, Kaoma Tony, Sapcariu Sean C., Dilger Marco, Paur Hanns-Rudolf, Schlager Christoph, Mülhopt Sonja, Weiss Carsten, Schmidt-Weber Carsten, Traidl-Hoffmann Claudia, Michalke Bernhard, Krebs Tobias, Karg Erwin, Jakobi Gert, Scholtes SoranaSchnelle-Kreis Jürgen, Sklorz Martin, Orasche Jürgen, Müller Laarnie, Reda Ahmed, Rüger Christopher, Neumann Anika, Abbaszade Gülcin, Radischat Christian, Hiller Karsten, Grigonyte Julija, Kortelainen Miika, Kuuspalo Kari, Lamberg Heikki, Leskinen Jani, Nuutinen Ilpo, Torvela Tiina, Tissari Jarkko, Jalava Pasi, Kasurinen Stefanie, Uski Oskari, Hirvonen Maija-Riitta, Buters Jeroen, Dittmar Gunnar, Jokiniemi Jorma K., Zimmermann Ralf

Research output: Contribution to journalArticleResearchpeer-review


The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects of wood smoke inhalation manifest in proinflammatory signaling, oxidative stress, DNA damage and hence increased cancer risk. In this study, the composition and acute biological impact of emissions of state-of-the-art wood combustion compliances: masonry heater (MH) and pellet boiler (PB) were investigated. Therefore A549 cells were exposed to emission aerosols in an automated air-liquid interface exposure station followed by cytotoxicity, transcriptome and proteome analyses. In parallel, aerosols were subjected to a chemical and physical characterization. Compared to PB, the MH combustion at the same dilution ratio resulted in a 3-fold higher particle mass concentration (PM2.5) and deposited dose (PB: 27 ± 2 ng/cm2 , MH; 73 ± 12 ng/cm2 ). Additionally, the MH aerosol displayed a substantially larger concentration of aldehydes, polycyclic aromatic hydrocarbons (PAH) or oxidized PAH. Gene ontology analysis of transcriptome of A549 cells exposed to MH emissions revealed the activation of pro-inflammatory response and key signaling cascades MAP kinase and JAK-STAT. Furthermore, CYP1A1, an essential enzyme in PAH metabolism, was induced. PB combustion aerosol activated the proinflammatory marker IL6 and different transport processes. The proteomics data uncovered induction of DNA damage-associated proteins in response to PB and DNA double-strand break processing proteins in response to MH emissions. Taking together, the MH produces emissions with a higher particle dose and more toxic compounds while causing only mild biological responses. This finding points to a significant mitigating effect of antioxidative compounds in MH wood smoke
Original languageEnglish
Pages (from-to)23-35
Number of pages13
Issue number1
Publication statusPublished - 20 Jan 2018


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