Effects of high and low single dose irradiation on glioma spheroid invasion into normal rat brain tissue in vitro

O. Engebraaten*, R. Schwachenwald, H. Valen, R. Bjerkvig, O. D. Laerum, E. O. Backlund

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)


The effects of radiation on direction on directional migration in monolayer cultures and brain tissue invasion by two glioblastoma celle lines (D-54 MG, D-247 MG) were investigated. The Leksell Gamma Unit was the radiation soucre and invasion was registered in an in vitro invasion assay developed in our laboratory. As tumor spheroids and brain tissue aggregates were treated simultaneously in cocultures; the effects of radiation on the interaction between the two tissues could be investigated. Tumor spheroids from both cell lines retained their ability to invade and destroy normal brain tissue, even after irradiation with 47.6 Gy. However, while the D-54 MG tumor spheroids showed a dose-dependent reduction of invasion, tumor spheroids from the D-247 MG cell line did not. In addition, radiation produced a dose dependent inhibition of directional migration of cells from D-54 MG spheroids. A similar significant inhibition of directional migration was found in D-247 MG, but it was not dose-dependent. Transsmission electron microscopy revealed a loosening of the neuropil in the brain tissue of irradiated cocultures. However, this structural change did not seem to affect the invasiveness of the tumor. In this preliminary study, irradiation could not prevent invasion of two different glioblastoma cell lines into fetal rat brain tissue. Further studies using the same technique may help to understand the influence of ionizing radiation upon the invasion process in gliomas.

Original languageEnglish
Pages (from-to)1501-1506
Number of pages6
JournalAnticancer Research
Issue number5
Publication statusPublished - 1992
Externally publishedYes


  • Glioma
  • Invasion
  • Leksell Gamma Unit
  • Radiation


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