Effect of open wedge high tibial osteotomy on the lateral compartment in sheep. Part I: Analysis of the lateral meniscus

Henning Madry*, Raphaela Ziegler, Patrick Orth, Lars Goebel, Mei Fang Ong, Dieter Kohn, Magali Cucchiarini, Dietrich Pape

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

Purpose: To evaluate whether medial open wedge high tibial osteotomy (HTO) results in structural and biochemical changes in the lateral meniscus in adult sheep. Methods: Three experimental groups with biplanar osteotomies of the right proximal tibiae were tested: (a) closing wedge HTO resulting in 4. 5° of tibial varus, (b) open wedge HTO resulting in 4. 5° of tibial valgus (standard correction) and (c) open wedge HTO resulting in 9. 5° of valgus (overcorrection), each of which was compared to the contralateral knees with normal limb axes. After 6 months, the lateral menisci were macroscopically and microscopically evaluated. The proteoglycan and DNA contents of the red-red and white-white zones of the anterior, middle and posterior third were determined. Results: Semiquantitative macroscopic and microscopic grading revealed no structural differences between groups. The red-red zone of the middle third of the lateral menisci of animals that underwent overcorrection exhibited a significant 0. 7-fold decrease in mean DNA contents compared with the control knee without HTO (P = 0. 012). Comparative estimation of the DNA and proteoglycan contents and proteoglycan/DNA ratios of all other parts and zones of the lateral menisci did not reveal significant differences between groups. Conclusion: Open wedge HTO does not lead to significant macroscopic and microscopic structural changes in the lateral meniscus after 6 months in vivo. Overcorrection significantly decreases the proliferative activity of the cells in the red-red zone of the middle third in the sheep model.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

Keywords

  • DNA content
  • HTO
  • Histology
  • Lateral meniscus
  • Macroscopic evaluation
  • Proteoglycan content
  • Type I collagen

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