Abstract
Purpose: To evaluate whether medial open wedge high tibial osteotomy (HTO) results in structural and biochemical changes in the lateral meniscus in adult sheep. Methods: Three experimental groups with biplanar osteotomies of the right proximal tibiae were tested: (a) closing wedge HTO resulting in 4. 5° of tibial varus, (b) open wedge HTO resulting in 4. 5° of tibial valgus (standard correction) and (c) open wedge HTO resulting in 9. 5° of valgus (overcorrection), each of which was compared to the contralateral knees with normal limb axes. After 6 months, the lateral menisci were macroscopically and microscopically evaluated. The proteoglycan and DNA contents of the red-red and white-white zones of the anterior, middle and posterior third were determined. Results: Semiquantitative macroscopic and microscopic grading revealed no structural differences between groups. The red-red zone of the middle third of the lateral menisci of animals that underwent overcorrection exhibited a significant 0. 7-fold decrease in mean DNA contents compared with the control knee without HTO (P = 0. 012). Comparative estimation of the DNA and proteoglycan contents and proteoglycan/DNA ratios of all other parts and zones of the lateral menisci did not reveal significant differences between groups. Conclusion: Open wedge HTO does not lead to significant macroscopic and microscopic structural changes in the lateral meniscus after 6 months in vivo. Overcorrection significantly decreases the proliferative activity of the cells in the red-red zone of the middle third in the sheep model.
Original language | English |
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Pages (from-to) | 39-48 |
Number of pages | 10 |
Journal | Knee Surgery, Sports Traumatology, Arthroscopy |
Volume | 21 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2013 |
Externally published | Yes |
Keywords
- DNA content
- HTO
- Histology
- Lateral meniscus
- Macroscopic evaluation
- Proteoglycan content
- Type I collagen