TY - JOUR
T1 - Effect of combinational arginase and canavanine treatment on normal human peripheral blood lymphocytes in vitro
AU - Chen, Oleh
AU - Kavalets, Bohdanna
AU - Barska, Marina
AU - Lyniv, Lilianna
AU - Vovk, Olena
AU - Sybirna, Natalia
AU - Stasyk, Oleh
PY - 2013
Y1 - 2013
N2 - Recombinant human arginase (rhARG)-based therapy to restrict arginine availability for malignant cells is a promising strategy to treat some cancers, including those of hematological origin. In the present study, we evaluated the action of combined rhARG treatment with canavanine (Cav) on proliferative potential and functional state of human peripheral blood lymphocytes (PBL) in vitro. We demonstrated that Cav in 0.1 mM concentration alone had no significant effect on proliferation and functional activity of normal activated PBL. Combined rhARG and Cav treatment inhibited proliferation and slightly promoted apoptosis in activated PBL but did not affect resting cells. However, the action of both drugs affected the ability of T cells for antigen activation. These results are important for further translational research on arginine-deprivation therapy. 2013
AB - Recombinant human arginase (rhARG)-based therapy to restrict arginine availability for malignant cells is a promising strategy to treat some cancers, including those of hematological origin. In the present study, we evaluated the action of combined rhARG treatment with canavanine (Cav) on proliferative potential and functional state of human peripheral blood lymphocytes (PBL) in vitro. We demonstrated that Cav in 0.1 mM concentration alone had no significant effect on proliferation and functional activity of normal activated PBL. Combined rhARG and Cav treatment inhibited proliferation and slightly promoted apoptosis in activated PBL but did not affect resting cells. However, the action of both drugs affected the ability of T cells for antigen activation. These results are important for further translational research on arginine-deprivation therapy. 2013
KW - Arginase
KW - Arginine deprivation
KW - Canavanine
KW - Peripheral blood lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=84896979913&partnerID=8YFLogxK
U2 - 10.12923/j.2084-980X/26.4/a.07
DO - 10.12923/j.2084-980X/26.4/a.07
M3 - Article
AN - SCOPUS:84896979913
SN - 2084-980X
VL - 26
SP - 385
EP - 389
JO - Current Issues in Pharmacy and Medical Sciences
JF - Current Issues in Pharmacy and Medical Sciences
IS - 4
ER -