Dopamine-induced conformational changes in alpha-synuclein

Tiago F. Outerio, Jochen Klucken, Kathryn Bercury, Julie Tetzlaff, Preeti Putcha, Luis M.A. Oliveira, Alexandre Quintas, Pamela J. McLean, Bradley T. Hyman

Research output: Contribution to journalArticleResearchpeer-review

57 Citations (Scopus)


Background: Oligomerization and aggregation of α-synuclein molecules play a major role in neuronal dysfunction and loss in Parkinson's disease. However, α-synuclein oligomerization and aggregation have mostly been detected indirectly in cells using detergent extraction methods. A number of in vitro studies showed that dopamine can modulate the aggregation of α-synuclein by inhibiting the formation of or by disaggregating amyloid fibrils. Methodology/Principal Findings: Here, we show that α-synuclein adopts a variety of conformations in primary neuronal cultures using fluorescence lifetime imaging microscopy (FLIM). Importantly, we found that dopamine, but not dopamine agonists, induced conformational changes in a-synuclein which could be prevented by blocking dopamine transport into the cell. Dopamine also induced conformational changes in α-synuclein expressed in neuronal cell lines, and these changes were also associated with alterations in oligomeric/aggregated species. Conclusion/Significance: Our results show, for the first time, a direct effect of dopamine on the conformation of α-synuclein in neurons, which may help explain the increased vulnerability of dopaminergic neurons in Parkinson's disease.

Original languageEnglish
Article numbere6906
JournalPLoS ONE
Issue number9
Publication statusPublished - 4 Sept 2009
Externally publishedYes


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