Divergent Expression of HLA-DC/MB, -DR, and -SB region products on normal and pathological tissues as detected by monoclonal antibodies

Claudia Müller, A. Ziegler, C. Müller, M. Hadam, H. D. Waller, P. Kernet, G. Müller

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29 Citations (Scopus)

Abstract

A group of eight monoclonal antibodies directed against different monomorphic determinantsof HLA-class II molecules was used to investigate the distribution of HLA-DC/DS/MB, -DR and -SB-like antigens on normal and pathological lymphoid and nonlymphoid tissues of human adult and fetal donors. HLA-MB/DC/DS-like molecules, as defined by the antibody TÜ 22, showed the most limited distribution as they were detected on B-lymphocytes, monocyte/macrophage subpopulations and distinct interstitial cells of various orpns. HLA-DR and -SB-like antigens characterized by the other anti-HLA-class II reagents (TÜ34, TÜ35, TÜ37, TÜ39, TÜ43, TÜ58) were also present on these cell types. However, selective expression of HLA-DR and/or -SB like molecules was demonstrated with these antibodies on certain vascular endothelia, as well as different B-cell lymphomas and distinct epithelial cells in adults. Exclusive reactivity of the antibody TÜ39 shown on endothelial cells of fetal liver and kidney suggested specific functions of HLA-SB antigens during ontogeny. Furthermore, HLA-DR and/or -SB like molecules but not TÜ22+ HLA-DC/MB antigens were found to be inducible on normally Ia-like antigen negative epithelial cells of various diseased organs. Implications of this differential tissue distribution of HLA-DC/MB/DS, -DR and -SB like products in relation to organ transplantation, regulation of immune responses and cell differentiation are discussed.

Original languageEnglish
Pages (from-to)228-249
Number of pages22
JournalImmunobiology
Volume169
Issue number3
DOIs
Publication statusPublished - 1985
Externally publishedYes

Keywords

  • CLL
  • FAGS
  • FMF
  • FWHM
  • Flow microfluorometry
  • HLA
  • MHC
  • chronic lymphocytic leukemia
  • fluorescence activated cell sorter
  • full width, half maximum
  • human leukocyte antigen
  • major histocompatibility complex

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