TY - JOUR
T1 - Divergent Expression of HLA-DC/MB, -DR, and -SB region products on normal and pathological tissues as detected by monoclonal antibodies
AU - Müller, Claudia
AU - Ziegler, A.
AU - Müller, C.
AU - Hadam, M.
AU - Waller, H. D.
AU - Kernet, P.
AU - Müller, G.
N1 - Funding Information:
Genetic, molecular and cellular analysis established the existence of at least three polymorphic cell membrane products (HLA-DR, -DC/DS/MB, -SB) encoded by different gene loci within the HLA-D region of the human major histocompatibility complex (MHC) (1-5). As homologues of the mouse Ia-(= immune-response associated) antigens, these molecules are * Supported by the DFG Sonderforschungsbereich 120 «Leukamieforschung und Immungenetih Projekt A3, A-4, and by the DFG Projekt Mu 523/3-1.
PY - 1985
Y1 - 1985
N2 - A group of eight monoclonal antibodies directed against different monomorphic determinantsof HLA-class II molecules was used to investigate the distribution of HLA-DC/DS/MB, -DR and -SB-like antigens on normal and pathological lymphoid and nonlymphoid tissues of human adult and fetal donors. HLA-MB/DC/DS-like molecules, as defined by the antibody TÜ 22, showed the most limited distribution as they were detected on B-lymphocytes, monocyte/macrophage subpopulations and distinct interstitial cells of various orpns. HLA-DR and -SB-like antigens characterized by the other anti-HLA-class II reagents (TÜ34, TÜ35, TÜ37, TÜ39, TÜ43, TÜ58) were also present on these cell types. However, selective expression of HLA-DR and/or -SB like molecules was demonstrated with these antibodies on certain vascular endothelia, as well as different B-cell lymphomas and distinct epithelial cells in adults. Exclusive reactivity of the antibody TÜ39 shown on endothelial cells of fetal liver and kidney suggested specific functions of HLA-SB antigens during ontogeny. Furthermore, HLA-DR and/or -SB like molecules but not TÜ22+ HLA-DC/MB antigens were found to be inducible on normally Ia-like antigen negative epithelial cells of various diseased organs. Implications of this differential tissue distribution of HLA-DC/MB/DS, -DR and -SB like products in relation to organ transplantation, regulation of immune responses and cell differentiation are discussed.
AB - A group of eight monoclonal antibodies directed against different monomorphic determinantsof HLA-class II molecules was used to investigate the distribution of HLA-DC/DS/MB, -DR and -SB-like antigens on normal and pathological lymphoid and nonlymphoid tissues of human adult and fetal donors. HLA-MB/DC/DS-like molecules, as defined by the antibody TÜ 22, showed the most limited distribution as they were detected on B-lymphocytes, monocyte/macrophage subpopulations and distinct interstitial cells of various orpns. HLA-DR and -SB-like antigens characterized by the other anti-HLA-class II reagents (TÜ34, TÜ35, TÜ37, TÜ39, TÜ43, TÜ58) were also present on these cell types. However, selective expression of HLA-DR and/or -SB like molecules was demonstrated with these antibodies on certain vascular endothelia, as well as different B-cell lymphomas and distinct epithelial cells in adults. Exclusive reactivity of the antibody TÜ39 shown on endothelial cells of fetal liver and kidney suggested specific functions of HLA-SB antigens during ontogeny. Furthermore, HLA-DR and/or -SB like molecules but not TÜ22+ HLA-DC/MB antigens were found to be inducible on normally Ia-like antigen negative epithelial cells of various diseased organs. Implications of this differential tissue distribution of HLA-DC/MB/DS, -DR and -SB like products in relation to organ transplantation, regulation of immune responses and cell differentiation are discussed.
KW - CLL
KW - FAGS
KW - FMF
KW - FWHM
KW - Flow microfluorometry
KW - HLA
KW - MHC
KW - chronic lymphocytic leukemia
KW - fluorescence activated cell sorter
KW - full width, half maximum
KW - human leukocyte antigen
KW - major histocompatibility complex
UR - http://www.scopus.com/inward/record.url?scp=0021922687&partnerID=8YFLogxK
U2 - 10.1016/S0171-2985(85)80036-X
DO - 10.1016/S0171-2985(85)80036-X
M3 - Article
AN - SCOPUS:0021922687
SN - 0171-2985
VL - 169
SP - 228
EP - 249
JO - Immunobiology
JF - Immunobiology
IS - 3
ER -