@article{0ecdb4e9eacf471e9b36964d9d914568,
title = "Dissecting the transcriptome in cardiovascular disease",
abstract = "The human transcriptome comprises a complex network of coding and non-coding RNAs implicated in a myriad of biological functions. Non-coding RNAs exhibit highly organized spatial and temporal expression patterns and are emerging as critical regulators of differentiation, homeostasis, and pathological states, including in the cardiovascular system. This review defines the current knowledge gaps, unmet methodological needs, and describes the challenges in dissecting and understanding the role and regulation of the non-coding transcriptome in cardiovascular disease. These challenges include poor annotation of the non-coding genome, determination of the cellular distribution of transcripts, assessment of the role of RNA processing and identification of cell-type specific changes in cardiovascular physiology and disease. We highlight similarities and differences in the hurdles associated with the analysis of the non-coding and protein-coding transcriptomes. In addition, we discuss how the lack of consensus and absence of standardized methods affect reproducibility of data. These shortcomings should be defeated in order to make significant scientific progress and foster the development of clinically applicable non-coding RNA-based therapeutic strategies to lessen the burden of cardiovascular disease.",
keywords = "Methodology standardisation, Non-coding RNAs, Transcriptomics, Translational cardiovascular research",
author = "Robinson, {Emma L.} and Baker, {Andrew H.} and Mairi Brittan and Ian McCracken and G. Condorelli and C. Emanueli and Srivastava, {P. K.} and C. Gaetano and T. Thum and M. Vanhaverbeke and C. Angione and S. Heymans and Y. Devaux and T. Pedrazzini and F. Martelli and {EU-CardioRNA COST Action CA17129}",
note = "E.L.R. is supported by a Cardiovasculair Onderzoek Nederland (CVON) RECONECT Talent programme grant (Dutch Heart Foundation). A.B. is supported by the European Research Council (VASCMIR-AB), the British Heart Foundation Chair of Translational Cardiovascular Sciences and Intermediate Basic Science Fellowship (MB), and I.M. by a Medical Research Council Doctoral Training Award in Precision Medicine. S.H. received funding from the CVON2016-Early HFPEF, CVON She- PREDICTS, and CVON-Arena-PRIME (Dutch Heart Foundation). C.E. is supported by a British Heart Foundation Programme Grant and Personal Chair Awards (RG/15/5/31446, CH/15/1/31199). T.T. is supported by a Deutsche Forschungsgemeinschaft and EU grant CardioReGenix. Y.D. is funded by the National Research Fund (grants # C14/BM/8225223 and C17/BM/11613033), the Ministry of Higher Education and Research, and the Fondation Coeur–Daniel Wagner of Luxembourg. T.P. received grants from the Swiss National Science Foundation (No. CRSII5-1_173738 and 31003A_182322). F.M. is supported by the Italian Ministry of Health (“Ricerca Corrente” and “5x1000”), by AFM-Telethon (#23054), and by Telethon Foundation (GGP19035A). F.M., C.E., Y.D., and A.B. are also funded by the EU Horizon 2020 Project COVIRNA. Publisher Copyright: {\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of the European Society of Cardiology.",
year = "2022",
month = mar,
day = "16",
doi = "10.1093/cvr/cvab117",
language = "English",
volume = "118",
pages = "1004--1019",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "4",
}