TY - JOUR
T1 - Digital PCR cluster predictor
T2 - a universal R-package and shiny app for the automated analysis of multiplex digital PCR data
AU - De Falco, Alfonso
AU - Olinger, Christophe M.
AU - Klink, Barbara
AU - Mittelbronn, Michel
AU - Stieber, Daniel
N1 - Funding
This work was supported by the Luxembourg National Research Fond (FNR) [PRIDE15/10675146/CANBIO, FNR PEARL P16/BM/11192868 to M.M.]
© The Author(s) 2023. Published by Oxford University Press.
PY - 2023/5/4
Y1 - 2023/5/4
N2 - Digital polymerase chain reaction (dPCR) is an emerging technology that enables accurate and sensitive quantification of nucleic acids. Most available dPCR systems have two channel optics, with ad hoc software limited to the analysis of single and duplex assays. Although multiplexing strategies were developed, variable assay designs, dPCR systems, and the analysis of low DNA input data restricted the ability for a universal automated clustering approach. To overcome these issues, we developed dPCR Cluster Predictor (dPCP), an R package and a Shiny app for automated analysis of up to 4-plex dPCR data. dPCP can analyse and visualize data generated by multiple dPCR systems carrying out accurate and fast clustering not influenced by the amount and integrity of input of nucleic acids. With the companion Shiny app, the functionalities of dPCP can be accessed through a web browser.
AB - Digital polymerase chain reaction (dPCR) is an emerging technology that enables accurate and sensitive quantification of nucleic acids. Most available dPCR systems have two channel optics, with ad hoc software limited to the analysis of single and duplex assays. Although multiplexing strategies were developed, variable assay designs, dPCR systems, and the analysis of low DNA input data restricted the ability for a universal automated clustering approach. To overcome these issues, we developed dPCR Cluster Predictor (dPCP), an R package and a Shiny app for automated analysis of up to 4-plex dPCR data. dPCP can analyse and visualize data generated by multiple dPCR systems carrying out accurate and fast clustering not influenced by the amount and integrity of input of nucleic acids. With the companion Shiny app, the functionalities of dPCP can be accessed through a web browser.
UR - http://www.scopus.com/inward/record.url?scp=85159729979&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/37086434
U2 - 10.1093/bioinformatics/btad282
DO - 10.1093/bioinformatics/btad282
M3 - Article
C2 - 37086434
SN - 1367-4803
VL - 39
JO - Bioinformatics
JF - Bioinformatics
IS - 5
ER -