TY - JOUR
T1 - Development of a multi-class method for the quantification of veterinary drug residues in feedingstuffs by liquid chromatography-tandem mass spectrometry
AU - Boscher, Aurore
AU - Guignard, Cédric
AU - Pellet, Terence
AU - Hoffmann, Lucien
AU - Bohn, Torsten
N1 - Funding Information:
This work was supported by the ASTA (Administration des Services Techniques de l’Agriculture in Luxembourg). We wish to express our sincere thanks to Mr. Navette from Versis for providing the feedingstuff samples, and Boris Untereiner for this contribution to the experiments.
PY - 2010/10
Y1 - 2010/10
N2 - A simple multi-residue method was developed for detecting and quantifying 33 analytes from 13 classes of antibiotics (tetracyclines (3), quinolones (7), penicillins (3), ionophore coccidiostats (7), macrolides (3), sulfonamides (1), quinoxalines (2), phenicols (2), lincosamides (1), diaminopyrimidines (1), polypeptides (1), streptogramins (1) and pleuromutilins (1)) in animal feeds. Extraction and clean-up procedures were optimized with spiked piglet feed. Samples were extracted by ultrasonic-assisted extraction with a mixture of methanol/acetonitrile/McIlvaine buffer at pH 4.6 (37.5/37.5/25, v/v/v) containing 0.3% of EDTA-Na2, followed by a clean up using a dispersive solid-phase extraction (d-SPE) with PSA (primary secondary amine). Detection of antibiotics was achieved by liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) within 28min using both positive and negative ESI mode. Average recoveries ranged from 51% (oxytetracycline) to 116% (tilmicosin) with associated relative standard deviations of 7.3% and 9.0% and an overall mean of 87%. Limits of quantification ranged from 3.8ngg-1 (lincomycin) to 65.0ngg-1 (bacitracin). Following optimization, the method was further verified for bovine and lamb feedingstuffs; negative matrix effects were evaluated and overcome by a standard addition method.
AB - A simple multi-residue method was developed for detecting and quantifying 33 analytes from 13 classes of antibiotics (tetracyclines (3), quinolones (7), penicillins (3), ionophore coccidiostats (7), macrolides (3), sulfonamides (1), quinoxalines (2), phenicols (2), lincosamides (1), diaminopyrimidines (1), polypeptides (1), streptogramins (1) and pleuromutilins (1)) in animal feeds. Extraction and clean-up procedures were optimized with spiked piglet feed. Samples were extracted by ultrasonic-assisted extraction with a mixture of methanol/acetonitrile/McIlvaine buffer at pH 4.6 (37.5/37.5/25, v/v/v) containing 0.3% of EDTA-Na2, followed by a clean up using a dispersive solid-phase extraction (d-SPE) with PSA (primary secondary amine). Detection of antibiotics was achieved by liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) within 28min using both positive and negative ESI mode. Average recoveries ranged from 51% (oxytetracycline) to 116% (tilmicosin) with associated relative standard deviations of 7.3% and 9.0% and an overall mean of 87%. Limits of quantification ranged from 3.8ngg-1 (lincomycin) to 65.0ngg-1 (bacitracin). Following optimization, the method was further verified for bovine and lamb feedingstuffs; negative matrix effects were evaluated and overcome by a standard addition method.
KW - Antibiotics
KW - Coccidiostats
KW - Feed
KW - LC-MS/MS
KW - Matrix effects
KW - Standard addition method
UR - http://www.scopus.com/inward/record.url?scp=77956776039&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2010.08.024
DO - 10.1016/j.chroma.2010.08.024
M3 - Article
C2 - 20810120
AN - SCOPUS:77956776039
SN - 0021-9673
VL - 1217
SP - 6394
EP - 6404
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 41
ER -