TY - JOUR
T1 - Determination of chlorophyll in plant samples by liquid chromatography using zinc-phthalocyanine as an internal standard
AU - Bohn, Torsten
AU - Walczyk, Thomas
PY - 2004/1/23
Y1 - 2004/1/23
N2 - Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a selection of vegetable foods. Pigments were extracted with N,N-dimethylformamide from the vegetables and purified by solid phase extraction. Chlorophyll a, a′, b, b′, corresponding pheophytins, and zinc-phthalocyanine were separated by HPLC using a C18 reverse-phase column and fluorescence detection.
AB - Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a selection of vegetable foods. Pigments were extracted with N,N-dimethylformamide from the vegetables and purified by solid phase extraction. Chlorophyll a, a′, b, b′, corresponding pheophytins, and zinc-phthalocyanine were separated by HPLC using a C18 reverse-phase column and fluorescence detection.
KW - Chlorophylls
KW - Zinc-phthalocyanine
UR - http://www.scopus.com/inward/record.url?scp=0346849986&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2003.10.067
DO - 10.1016/j.chroma.2003.10.067
M3 - Article
C2 - 14753714
AN - SCOPUS:0346849986
SN - 0021-9673
VL - 1024
SP - 123
EP - 128
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -