Detection and Discovery of Coronaviruses in Wild Bird Populations

Chantal J. Snoeck*, Siamak Zohari

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughput sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Deltacoronavirus
  • Gammacoronavirus
  • Illumina MiSeq
  • Next-generation sequencing
  • Pan-Coronavirus
  • RT-PCR
  • Sample collection
  • Wild birds


Dive into the research topics of 'Detection and Discovery of Coronaviruses in Wild Bird Populations'. Together they form a unique fingerprint.

Cite this