Data on European seabass fed with methionine-enriched diets obtained through label free shotgun proteomics

Ana Paula Farinha*, Denise Schrama, Tomé Silva, Luís E.C. Conceição, Rita Colen, Sofia Engrola, Pedro Rodrigues, Marco Cerqueira

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)


This data article is associated with the research article “Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics”. Here it is described the data obtained from proteomic analysis of 36 European seabass juveniles (3 fish x 3 replicate tanks) after 18 days of feeding with experimental diets containing four inclusion levels of methionine (Met): 0.77%, 1%, 1.36% and 1.66% Met (w/w). We analysed this dataset and compared it with that obtained during the long-term feeding period i.e., 85 days. Fish liver proteins were digested with trypsin and purified peptides were analysed by LC-MS/MS. Proteins were identified with at least two peptides at 0.1% Decoy false discovery rate (FDR). In this dataset, we present the analysis of the differential abundant proteins (DAP) with significant differences across treatments after 18 days of feeding (One-Way ANOVA, p < 0.05). Treatment's comparisons were also performed between the 18- and 85-days feeding trials through Two-Way ANOVA (p < 0.05). MS/MS raw data are available via ProteomeXChange with identifiers PXD019610 and 10.6019/PXD019610 (18-days dataset); and PXD019622 and 10.6019/PXD019622 (85-days dataset). This dataset corresponds to fish sampled after 18-days of experimental trial and is made available to support the study conducted in the afore-mentioned article, by performing the analysis during a short-term period of feeding. The data presented may be further used in other nutritional studies e.g., addressing hepatic changes mediated by Met.

Original languageEnglish
Article number106675
JournalData in Brief
Publication statusPublished - Feb 2021
Externally publishedYes


  • Dicentrarchus labrax
  • Farmed fish
  • Liver proteome
  • Metabolism
  • Methionine
  • Nutritional proteomics


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