Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

A. Peláez-García; R. Barderas; M. Mendes; M. Lopez-Lucendo; J. C. Sanchez; A. García de Herreros; J. I. Casal

doi:10.1016/j.dib.2015.07.027

Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

A. Peláez-García, R. Barderas, M. Mendes, M. Lopez-Lucendo, J. C. Sanchez, A. García de Herreros, J. I. Casal^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

Original language	English
Pages (from-to)	606-613
Number of pages	8
Journal	Data in Brief
Volume	4
DOIs	https://doi.org/10.1016/j.dib.2015.07.027
Publication status	Published - 1 Sept 2015
Externally published	Yes

Keywords

Adipogenesis
Differentiation
IL-17
Mesenchymal cells
Obesity
Proteomics
Snail
Transcription factors

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title = "Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation",

abstract = "The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].",

keywords = "Adipogenesis, Differentiation, IL-17, Mesenchymal cells, Obesity, Proteomics, Snail, Transcription factors",

author = "A. Pel{\'a}ez-Garc{\'i}a and R. Barderas and M. Mendes and M. Lopez-Lucendo and Sanchez, {J. C.} and {Garc{\'i}a de Herreros}, A. and Casal, {J. I.}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

month = sep,

day = "1",

doi = "10.1016/j.dib.2015.07.027",

language = "English",

volume = "4",

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T1 - Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

AU - Peláez-García, A.

AU - Barderas, R.

AU - Mendes, M.

AU - Lopez-Lucendo, M.

AU - Sanchez, J. C.

AU - García de Herreros, A.

AU - Casal, J. I.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

AB - The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

KW - Adipogenesis

KW - Differentiation

KW - IL-17

KW - Mesenchymal cells

KW - Obesity

KW - Proteomics

KW - Snail

KW - Transcription factors

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U2 - 10.1016/j.dib.2015.07.027

DO - 10.1016/j.dib.2015.07.027

M3 - Article

AN - SCOPUS:84939504351

SN - 2352-3409

VL - 4

SP - 606

EP - 613

JO - Data in Brief

JF - Data in Brief

ER -

Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

Abstract

Keywords

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