TY - JOUR
T1 - Daily rhythms of heart rate, temperature and locomotor activity are modified by anaesthetics in rats
T2 - A telemetric study
AU - Prudian, Frederic
AU - Gantenbein, Manon
AU - Pelissier, Anne Laure
AU - Attolini, Laurence
AU - Bruguerolle, Bernard
N1 - Funding Information:
Acknowledgements This work was partly supported by a grant from DGRT (UPRESS EA 2199) Mediterranean University (Aix-Marseilles II), Faculty of Medicine of Marseilles, France. Special thanks to J. Mouchet for technical assistance. The authors want to thank E. Jouve for her precious advices in statistics.
PY - 1997
Y1 - 1997
N2 - The aim of this study was to evaluate the effect of anaesthesia (ether or ketamine) on daily rhythms of temperature (T), heart rate (H) and locomotor activity (A) in unrestrained rats by using implanted radio-telemetry transmitters. T, H and A were measured every 10 min, in Wistar male rats, and analysed using Cosinor. The mean±SEM days needed, after surgical implantation, to detect a daily rhythm in H, T and A were also assessed. Six rats were anaesthetized for about 50 min either by ketamine or ether in a 3 by 3 cross-over design. Mesors, amplitudes and acrophases of T, H and A were calculated three days before (D-3; D-2; D-1), the day of anaesthesia (DO) as well as the three following days (D1; D2; D3). ANOVA was performed in order to detect, firstly a possible effect due to the order of application of anaesthesia, secondly a significant difference between ether or ketamine-induced anaesthesia and finally a modification of the mesors, amplitudes and acrophases of T, H and A, induced by each anaesthesia, for DO, D1, D2 and D3 when compared to D-1. Our results indicate: (1) Alterations of the acrophases, mesors and amplitudes, except for the amplitude of A, of the daily rhythms of T, H and A on DO of ketamine anaesthesia while regarding ether anaesthesia only amplitude of T and H and acrophase of A were modified on DO. Some of these modifications were still observed on the days following anaesthesia. A significant difference between ether and ketamine-induced anaesthesia was also observed. (2) A non-detection of T, H and A daily rhythms after surgical implantation, which was not observed after injection of either ether or ketamine alone. Almost 10 days were needed to detect a significant daily rhythm for T, H and A. The authors suggest that, the general anaesthetic agent was responsible for a perturbation of the mesors, amplitudes and acrophases of the daily rhythms of H, T and A while the non-detection of these rhythms after implantation was more due to the surgical aggression.
AB - The aim of this study was to evaluate the effect of anaesthesia (ether or ketamine) on daily rhythms of temperature (T), heart rate (H) and locomotor activity (A) in unrestrained rats by using implanted radio-telemetry transmitters. T, H and A were measured every 10 min, in Wistar male rats, and analysed using Cosinor. The mean±SEM days needed, after surgical implantation, to detect a daily rhythm in H, T and A were also assessed. Six rats were anaesthetized for about 50 min either by ketamine or ether in a 3 by 3 cross-over design. Mesors, amplitudes and acrophases of T, H and A were calculated three days before (D-3; D-2; D-1), the day of anaesthesia (DO) as well as the three following days (D1; D2; D3). ANOVA was performed in order to detect, firstly a possible effect due to the order of application of anaesthesia, secondly a significant difference between ether or ketamine-induced anaesthesia and finally a modification of the mesors, amplitudes and acrophases of T, H and A, induced by each anaesthesia, for DO, D1, D2 and D3 when compared to D-1. Our results indicate: (1) Alterations of the acrophases, mesors and amplitudes, except for the amplitude of A, of the daily rhythms of T, H and A on DO of ketamine anaesthesia while regarding ether anaesthesia only amplitude of T and H and acrophase of A were modified on DO. Some of these modifications were still observed on the days following anaesthesia. A significant difference between ether and ketamine-induced anaesthesia was also observed. (2) A non-detection of T, H and A daily rhythms after surgical implantation, which was not observed after injection of either ether or ketamine alone. Almost 10 days were needed to detect a significant daily rhythm for T, H and A. The authors suggest that, the general anaesthetic agent was responsible for a perturbation of the mesors, amplitudes and acrophases of the daily rhythms of H, T and A while the non-detection of these rhythms after implantation was more due to the surgical aggression.
KW - Anaesthesia
KW - Daily rhythms
KW - Ether
KW - Heart rate
KW - Ketamine
KW - Locomotor activity
KW - Rat
KW - Telemetry
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=0030827736&partnerID=8YFLogxK
U2 - 10.1007/PL00005012
DO - 10.1007/PL00005012
M3 - Article
C2 - 9205963
AN - SCOPUS:0030827736
SN - 0028-1298
VL - 355
SP - 774
EP - 778
JO - Naunyn-Schmiedeberg's Archives of Pharmacology
JF - Naunyn-Schmiedeberg's Archives of Pharmacology
IS - 6
ER -