TY - JOUR
T1 - Cytokeratin 18 expression pattern correlates with renal cell carcinoma progression
T2 - Relationship with Snail
AU - Messai, Yosra
AU - Noman, Muhammad Zaeem
AU - Derouiche, Amine
AU - Kourda, Nadia
AU - Akalay, Intissar
AU - Hasmim, Meriem
AU - Stasik, Izabela
AU - Jilani, Sarra Ben
AU - Chebil, Mohamed
AU - Caignard, Anne
AU - Azzarone, Bruno
AU - Gati, Asma
AU - Elgaaied, Amel Ben Ammar
AU - Chouaib, Salem
PY - 2010/5
Y1 - 2010/5
N2 - Renal cell carcinoma (RCC) is the most common type of kidney cancer and recent developments in the molecular biology of RCC have identified multiple pathways associated with the development of this cancer. This study aimed at analyzing the expression pattern of cytokeratin 18 (CK18) in RCC patients and its prognostic relevance. We quantified CK18 mRNA expression and protein using real-time reverse transcription quantitative polymerase chain reaction (RTQPCR) and immunohistochemistry, respectively, in paired tumor and non-tumor samples from 42 patients. Our data indicate that CK18 mRNA and proteins levels increased with advanced stage and grade of the disease. Using primary (RCC5) and metastatic renal cell carcinoma (RCC5 met) cell lines, we demonstrated that CK18 expression was 5-fold higher in the metastatic as compared to the primary RCC cell line and correlated with a migratory phenotype characterized by a distinct elongated morphology as revealed by Phalloidin staining. In addition, RCC5 met cells displayed an increased capacity to attach to fibronectin and collagen which was lost following CK18 knock-down. Our data also indicate that the expression of CK18 was associated with increased Snail expression which correlated positively with advanced disease in RCC patients. The present findings suggest that CK18 may play an important role in the progression of RCC and it may be used as a new predictor for RCC.
AB - Renal cell carcinoma (RCC) is the most common type of kidney cancer and recent developments in the molecular biology of RCC have identified multiple pathways associated with the development of this cancer. This study aimed at analyzing the expression pattern of cytokeratin 18 (CK18) in RCC patients and its prognostic relevance. We quantified CK18 mRNA expression and protein using real-time reverse transcription quantitative polymerase chain reaction (RTQPCR) and immunohistochemistry, respectively, in paired tumor and non-tumor samples from 42 patients. Our data indicate that CK18 mRNA and proteins levels increased with advanced stage and grade of the disease. Using primary (RCC5) and metastatic renal cell carcinoma (RCC5 met) cell lines, we demonstrated that CK18 expression was 5-fold higher in the metastatic as compared to the primary RCC cell line and correlated with a migratory phenotype characterized by a distinct elongated morphology as revealed by Phalloidin staining. In addition, RCC5 met cells displayed an increased capacity to attach to fibronectin and collagen which was lost following CK18 knock-down. Our data also indicate that the expression of CK18 was associated with increased Snail expression which correlated positively with advanced disease in RCC patients. The present findings suggest that CK18 may play an important role in the progression of RCC and it may be used as a new predictor for RCC.
KW - CK18
KW - Renal cell carcinoma
KW - Snail
KW - Tumor progression
UR - http://www.scopus.com/inward/record.url?scp=77950406581&partnerID=8YFLogxK
U2 - 10.3892/ijo-00000597
DO - 10.3892/ijo-00000597
M3 - Article
C2 - 20372788
AN - SCOPUS:77950406581
SN - 1019-6439
VL - 36
SP - 1145
EP - 1154
JO - International Journal of Oncology
JF - International Journal of Oncology
IS - 5
ER -