TY - JOUR
T1 - Curcumin stability and its effect on glutathione S-transferase P1-1 mRNA expression in K562 cells
AU - Blasius, Romain
AU - Duvoix, Annelyse
AU - Morceau, Franck
AU - Schnekenburger, Michaël
AU - Delhalle, Sylvie
AU - Henry, Estelle
AU - Dicato, Mario
AU - Diederich, Marc
PY - 2004
Y1 - 2004
N2 - To investigate the stability of curcumin in physiological media, the absorption variation of a curcumin solution was measured in 0.1% and 10% FCS. Under daylight conditions, curcumin degraded very rapidly in 0.1% FCS and was found to be more stable in higher serum concentrations. Under dark conditions, almost no decomposition could be observed after 2 h, whether the measurements were performed in 0.1% or 10% FCS. Furthermore, depending on the medium concentration, differential glutathione S-transferase P1-1 mRNA expression could be observed in K562 cells after incubation with curcumin. Indeed, incubation in 0.1% FCS led to a decrease of mRNA expression, whereas incubation in 10% FCS induced an increase of mRNA production.
AB - To investigate the stability of curcumin in physiological media, the absorption variation of a curcumin solution was measured in 0.1% and 10% FCS. Under daylight conditions, curcumin degraded very rapidly in 0.1% FCS and was found to be more stable in higher serum concentrations. Under dark conditions, almost no decomposition could be observed after 2 h, whether the measurements were performed in 0.1% or 10% FCS. Furthermore, depending on the medium concentration, differential glutathione S-transferase P1-1 mRNA expression could be observed in K562 cells after incubation with curcumin. Indeed, incubation in 0.1% FCS led to a decrease of mRNA expression, whereas incubation in 10% FCS induced an increase of mRNA production.
KW - Curcumin
KW - Degradation
KW - Drug resistance
KW - GSTP1-1
KW - Leukemia
UR - http://www.scopus.com/inward/record.url?scp=15044361882&partnerID=8YFLogxK
U2 - 10.1196/annals.1329.055
DO - 10.1196/annals.1329.055
M3 - Article
C2 - 15659828
AN - SCOPUS:15044361882
SN - 0077-8923
VL - 1030
SP - 442
EP - 448
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -