Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

Claude P. Muller; Gert Krabichler

doi:10.1063/1.1140053

Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

Claude P. Muller^*, Gert Krabichler

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The usefulness and limitations of the Becton-Dickinson fluorescence- activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.

Original language	English
Pages (from-to)	1928-1932
Number of pages	5
Journal	Review of Scientific Instruments
Volume	59
Issue number	9
DOIs	https://doi.org/10.1063/1.1140053
Publication status	Published - 1988
Externally published	Yes

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title = "Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter",

abstract = "The usefulness and limitations of the Becton-Dickinson fluorescence- activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.",

author = "Muller, {Claude P.} and Gert Krabichler",

year = "1988",

doi = "10.1063/1.1140053",

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journal = "Review of Scientific Instruments",

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AU - Krabichler, Gert

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AB - The usefulness and limitations of the Becton-Dickinson fluorescence- activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.

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U2 - 10.1063/1.1140053

DO - 10.1063/1.1140053

M3 - Article

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VL - 59

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EP - 1932

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

IS - 9

ER -

Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

Abstract

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