TY - JOUR
T1 - Critical appraisal of the side population assay in stem cell and cancer stem cell research
AU - Golebiewska, Anna
AU - Brons, Nicolaas H.C.
AU - Bjerkvig, Rolf
AU - Niclou, Simone P.
PY - 2011/2/4
Y1 - 2011/2/4
N2 - The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
AB - The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
UR - http://www.scopus.com/inward/record.url?scp=79551602468&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2011.01.007
DO - 10.1016/j.stem.2011.01.007
M3 - Article
AN - SCOPUS:79551602468
SN - 1934-5909
VL - 8
SP - 136
EP - 147
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 2
ER -