Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study host–microbe cross talk

Alex Steimle*, Alessandro De Sciscio, Mareike Neumann, Erica Grant, Gabriel V. Pereira, Hiroshi Ohno, Eric C. Martens, Mahesh S. Desai

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Reproducible in vivo models are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characterized human gut species and how to verify their proper colonization in germ-free mice. This protocol can be modified to add or remove certain species of interest to investigate microbial mechanistic details in various disease models. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

Original languageEnglish
Article number100607
Pages (from-to)100607
JournalSTAR Protocols
Volume2
Issue number2
DOIs
Publication statusPublished - 18 Jun 2021

Keywords

  • Immunology
  • Microbiology
  • Model Organisms

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