@article{e9f900c4a91b4d6ab2d6819aada47c21,
title = "Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study host–microbe cross talk",
abstract = "Reproducible in vivo models are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characterized human gut species and how to verify their proper colonization in germ-free mice. This protocol can be modified to add or remove certain species of interest to investigate microbial mechanistic details in various disease models. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).",
keywords = "Immunology, Microbiology, Model Organisms",
author = "Alex Steimle and {De Sciscio}, Alessandro and Mareike Neumann and Erica Grant and Pereira, {Gabriel V.} and Hiroshi Ohno and Martens, {Eric C.} and Desai, {Mahesh S.}",
note = "Funding Information: Work in the authors? laboratory was supported by the following grants to M.S.D.: Luxembourg National Research Fund (FNR) CORE grants (C15/BM/10318186 and C18/BM/12585940), Personalized Medicine Consortium of Luxembourg Pump Prime grant (Die-IBD; PMC/2016/01), and FNR INTER Mobility grant (16/11455695). M.N. was supported by the FNR AFR bilateral grant (15/11228353). E.T.G. was supported by the FNR PRIDE grant (PRIDE17/11823097) and the Fondation du P?lican de Mie et Pierre Hippert-Faber, under the aegis of the Fondation de Luxembourg. Conceptualization, A.S. E.C.M. and M.S.D.; methodology, A.S. A.D.S. M.N. E.T.G. G.V.P. and M.S.D.; investigation, A.S. E.T.G. H.O. and M.S.D.; resources, A.S. G.V.P. E.C.M. and M.S.D.; writing ? original draft, A.S. and A.D.S.; writing ? review & editing, A.S. A.D.S. M.N. E.T.G. G.V.P. and M.S.D.; supervision, M.S.D.; funding acquisition, M.S.D. The authors declare no competing interests. Funding Information: Work in the authors{\textquoteright} laboratory was supported by the following grants to M.S.D.: Luxembourg National Research Fund (FNR) CORE grants ( C15/BM/10318186 and C18/BM/12585940 ), Personalized Medicine Consortium of Luxembourg Pump Prime grant (Die-IBD; PMC/2016/01 ), and FNR INTER Mobility grant ( 16/11455695 ). M.N. was supported by the FNR AFR bilateral grant ( 15/11228353 ). E.T.G. was supported by the FNR PRIDE grant ( PRIDE17/11823097 ) and the Fondation du P{\'e}lican de Mie et Pierre Hippert-Faber , under the aegis of the Fondation de Luxembourg. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100607",
language = "English",
volume = "2",
pages = "100607",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}