TY - JOUR
T1 - Considerations on selected reaction monitoring experiments
T2 - Implications for the selectivity and accuracy of measurements
AU - Domon, Bruno
PY - 2012/12
Y1 - 2012/12
N2 - Targeted MS analyses based on selected reaction monitoring (SRM) has enabled significant achievements in proteomic quantification, such that its application to clinical studies has augured great advancements for life sciences. The approach has been challenged by the complexity of clinical samples that affects the selectivity of measurements, in many cases limiting analytical performances to a larger extent than expected. This Personal Perspective discusses some insight to better comprehend the mismatch between the often underestimated sample complexity and the selectivity of SRM measurements performed on a triple quadrupole instrument. The implications for the design and evaluation of SRM assays are discussed and illustrated with selected examples, providing a baseline for a more critical use of the technique in the context of clinical samples and to evaluate alternative methods.
AB - Targeted MS analyses based on selected reaction monitoring (SRM) has enabled significant achievements in proteomic quantification, such that its application to clinical studies has augured great advancements for life sciences. The approach has been challenged by the complexity of clinical samples that affects the selectivity of measurements, in many cases limiting analytical performances to a larger extent than expected. This Personal Perspective discusses some insight to better comprehend the mismatch between the often underestimated sample complexity and the selectivity of SRM measurements performed on a triple quadrupole instrument. The implications for the design and evaluation of SRM assays are discussed and illustrated with selected examples, providing a baseline for a more critical use of the technique in the context of clinical samples and to evaluate alternative methods.
KW - Peptide quantification
KW - Selected reaction monitoring
KW - Selectivity
KW - Targeted proteomics
UR - http://www.scopus.com/inward/record.url?scp=84871420249&partnerID=8YFLogxK
U2 - 10.1002/prca.201200111
DO - 10.1002/prca.201200111
M3 - Article
C2 - 23112131
AN - SCOPUS:84871420249
SN - 1862-8346
VL - 6
SP - 609
EP - 614
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
IS - 11-12
ER -