Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009

Sylvain Skraber; Jérémie Langlet; Jacques R. Kremer; Joël Mossong; Sébastien De Landtsheer; Jos Even; Claude P. Muller; Lucien Hoffmann; Henry Michel Cauchie

doi:10.1128/AEM.00632-11

Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009

Sylvain Skraber
, Jérémie Langlet
, Jacques R. Kremer
, Joël Mossong
, Sébastien De Landtsheer
, Jos Even
, Claude P. Muller
, Lucien Hoffmann
, Henry Michel Cauchie^*

^*Corresponding author for this work

Department of Infection and Immunity

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

Original language	English
Pages (from-to)	5566-5568
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	15
DOIs	https://doi.org/10.1128/AEM.00632-11
Publication status	Published - Aug 2011

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title = "Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009",

abstract = "Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.",

author = "Sylvain Skraber and J{\'e}r{\'e}mie Langlet and Kremer, \{Jacques R.\} and Jo{\"e}l Mossong and \{De Landtsheer\}, S{\'e}bastien and Jos Even and Muller, \{Claude P.\} and Lucien Hoffmann and Cauchie, \{Henry Michel\}",

year = "2011",

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T1 - Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009

AU - Skraber, Sylvain

AU - Langlet, Jérémie

AU - Kremer, Jacques R.

AU - Mossong, Joël

AU - De Landtsheer, Sébastien

AU - Even, Jos

AU - Muller, Claude P.

AU - Hoffmann, Lucien

AU - Cauchie, Henry Michel

PY - 2011/8

Y1 - 2011/8

N2 - Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

AB - Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

UR - https://www.scopus.com/pages/publications/79961065574

U2 - 10.1128/AEM.00632-11

DO - 10.1128/AEM.00632-11

M3 - Article

C2 - 21705540

AN - SCOPUS:79961065574

SN - 0099-2240

VL - 77

SP - 5566

EP - 5568

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 15

ER -

Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009

Abstract

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