TY - JOUR
T1 - Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009
AU - Skraber, Sylvain
AU - Langlet, Jérémie
AU - Kremer, Jacques R.
AU - Mossong, Joël
AU - De Landtsheer, Sébastien
AU - Even, Jos
AU - Muller, Claude P.
AU - Hoffmann, Lucien
AU - Cauchie, Henry Michel
PY - 2011/8
Y1 - 2011/8
N2 - Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.
AB - Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.
UR - http://www.scopus.com/inward/record.url?scp=79961065574&partnerID=8YFLogxK
U2 - 10.1128/AEM.00632-11
DO - 10.1128/AEM.00632-11
M3 - Article
C2 - 21705540
AN - SCOPUS:79961065574
SN - 0099-2240
VL - 77
SP - 5566
EP - 5568
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 15
ER -