TY - JOUR
T1 - Component-resolved evaluation of the content of major allergens in therapeutic extracts for specific immunotherapy of honeybee venom allergy
AU - Blank, Simon
AU - Etzold, Stefanie
AU - Darsow, Ulf
AU - Schiener, Maximilian
AU - Eberlein, Bernadette
AU - Russkamp, Dennis
AU - Wolf, Sara
AU - Graessel, Anke
AU - Biedermann, Tilo
AU - Ollert, Markus
AU - Schmidt-Weber, Carsten B.
N1 - Publisher Copyright:
© 2017 The Author(s). Published with license by Taylor & Francis © 2017, © Simon Blank, Stefanie Etzold, Ulf Darsow, Maximilian Schiener, Bernadette Eberlein, Dennis Russkamp, Sara Wolf, Anke Graessel, Tilo Biedermann, Markus Ollert, and Carsten B. Schmidt-Weber.
PY - 2017/10/3
Y1 - 2017/10/3
N2 - Allergen-specific immunotherapy is the only curative treatment of honeybee venom (HBV) allergy, which is able to protect against further anaphylactic sting reactions. Recent analyses on a molecular level have demonstrated that HBV represents a complex allergen source that contains more relevant major allergens than formerly anticipated. Moreover, allergic patients show very diverse sensitization profiles with the different allergens. HBV-specific immunotherapy is conducted with HBV extracts which are derived from pure venom. The allergen content of these therapeutic extracts might differ due to natural variations of the source material or different down-stream processing strategies of the manufacturers. Since variations of the allergen content of therapeutic HBV extracts might be associated with therapeutic failure, we adressed the component-resolved allergen composition of different therapeutic grade HBV extracts which are approved for immunotherapy in numerous countries. The extracts were analyzed for their content of the major allergens Api m 1, Api m 2, Api m 3, Api m 5 and Api m 10. Using allergen-specific antibodies we were able to demonstrate the underrepresentation of relevant major allergens such as Api m 3, Api m 5 and Api m 10 in particular therapeutic extracts. Taken together, standardization of therapeutic extracts by determination of the total allergenic potency might imply the intrinsic pitfall of losing information about particular major allergens. Moreover, the variable allergen composition of different therapeutic HBV extracts might have an impact on therapy outcome and the clinical management of HBV-allergic patients with specific IgE to particular allergens.
AB - Allergen-specific immunotherapy is the only curative treatment of honeybee venom (HBV) allergy, which is able to protect against further anaphylactic sting reactions. Recent analyses on a molecular level have demonstrated that HBV represents a complex allergen source that contains more relevant major allergens than formerly anticipated. Moreover, allergic patients show very diverse sensitization profiles with the different allergens. HBV-specific immunotherapy is conducted with HBV extracts which are derived from pure venom. The allergen content of these therapeutic extracts might differ due to natural variations of the source material or different down-stream processing strategies of the manufacturers. Since variations of the allergen content of therapeutic HBV extracts might be associated with therapeutic failure, we adressed the component-resolved allergen composition of different therapeutic grade HBV extracts which are approved for immunotherapy in numerous countries. The extracts were analyzed for their content of the major allergens Api m 1, Api m 2, Api m 3, Api m 5 and Api m 10. Using allergen-specific antibodies we were able to demonstrate the underrepresentation of relevant major allergens such as Api m 3, Api m 5 and Api m 10 in particular therapeutic extracts. Taken together, standardization of therapeutic extracts by determination of the total allergenic potency might imply the intrinsic pitfall of losing information about particular major allergens. Moreover, the variable allergen composition of different therapeutic HBV extracts might have an impact on therapy outcome and the clinical management of HBV-allergic patients with specific IgE to particular allergens.
KW - Api m 10
KW - Api m 3
KW - Api m 5
KW - allergen content
KW - allergen-specific antibody
KW - allergen-specific immunotherapy
KW - honeybee venom allergy
KW - hymenoptera venom
KW - venom extract
KW - venom immunotherapy
UR - http://www.scopus.com/inward/record.url?scp=85020618950&partnerID=8YFLogxK
UR - https://pubmed.nci.nlm.nih.gov/28494206/
U2 - 10.1080/21645515.2017.1323603
DO - 10.1080/21645515.2017.1323603
M3 - Article
C2 - 28494206
AN - SCOPUS:85020618950
SN - 2164-5515
VL - 13
SP - 2482
EP - 2489
JO - Human Vaccines and Immunotherapeutics
JF - Human Vaccines and Immunotherapeutics
IS - 10
ER -