@article{10fb682cfc1f4142911a9177f1210116,
title = "Cold Ischemia Score: An mRNA Assay for the Detection of Extended Cold Ischemia in Formalin-Fixed, Paraffin-Embedded Tissue",
abstract = "Although there are thousands of formalin-fixed paraffin-embedded (FFPE) tissue blocks potentially available for scientific research, many are of questionable quality, partly due to unknown preanalytical variables. We analyzed FFPE tissue biospecimens as part of the National Cancer Institute (NCI) Biospecimen Preanalytical Variables program to identify mRNA markers denoting cold ischemic time. The mRNA was extracted from colon, kidney, and ovary cancer FFPE blocks (40 patients, 10–12 hr fixation time) with 1, 2, 3, and 12 hr cold ischemic times, then analyzed using qRT-PCR for 23 genes selected following a literature search. No genes tested could determine short ischemic times (1–3 hr). However, a combination of three unstable genes normalized to a more stable gene could generate a “Cold Ischemia Score” that could distinguish 1 to 3 hr cold ischemia from 12 hr cold ischemia with 62% sensitivity and 84% specificity.",
keywords = "EGR1, FOS, POLR2A, PRKACA, cancer, degradation, stability, transcript",
author = "William Mathieson and Kathleen Mommaerts and Trouet, {Johanna M.} and Conny Mathay and Ping Guan and Carithers, {Latarsha J.} and Daniel Rohrer and Valley, {Dana R.} and Alexander Blanski and Scott Jewell and Moore, {Helen M.} and Fay Betsou",
note = "Funding Information: We acknowledge the valuable assistance provided by Petr Nazarov in respect of the statistical analyses using R and Monica Marchese for compiling the Receiver Operating Characteristic (ROC) curves. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Funds for the collection, processing, and RNA extraction of the biospecimens was provided by the Biospecimen Preanalytical Variables (BPV) Program of the Biorepositories and Biospecimen Research Branch of the National Institutes of Health, USA. The qRT-PCR, data analyses, and publication of the manuscript was funded by the Integrated Biobank of Luxembourg. Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Funds for the collection, processing, and RNA extraction of the biospecimens was provided by the Biospecimen Preanalytical Variables (BPV) Program of the Biorepositories and Biospecimen Research Branch of the National Institutes of Health, USA. The qRT-PCR, data analyses, and publication of the manuscript was funded by the Integrated Biobank of Luxembourg. Publisher Copyright: {\textcopyright} The Author(s) 2018.",
year = "2019",
month = mar,
day = "1",
doi = "10.1369/0022155418819967",
language = "English",
volume = "67",
pages = "159--168",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "3",
}