Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei

Matthias Dormeyer, Ralf Schöneck, Gunnar A.G. Dittmar, R. Luise Krauth-Siegel*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR (nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coli resulted in 2-5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite.

Original languageEnglish
Pages (from-to)449-453
Number of pages5
JournalFEBS Letters
Volume414
Issue number2
DOIs
Publication statusPublished - 8 Sep 1997
Externally publishedYes

Keywords

  • Drug target
  • Ribonucleotide reductase
  • Trypanosoma brucei
  • Trypanothione

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