TY - JOUR
T1 - Clinical characteristics of women diagnosed with carcinoma who tested positive for cervical and anal high-risk human papillomavirus DNA and E6 RNA
AU - Veo, Carlos A.R.
AU - Saad, Sarhan S.
AU - Fregnani, José Humberto T.G.
AU - Scapulatempo-Neto, Cristovam
AU - Tsunoda, Audrey Tieko
AU - Resende, Júlio César Possati
AU - Lorenzi, Adriana Tarlá
AU - Mafra, Allini
AU - Cinti, Claudia
AU - Cotrim, Ismael Dale
AU - Rosa, Luciana Albina Reis
AU - de Oliveira, Cristina Mendes
AU - Martins, Toni Ricardo
AU - Centrone, Cristiane
AU - Levi, José Eduardo
AU - Longatto-Filho, Adhemar
N1 - Publisher Copyright:
© 2015, International Society of Oncology and BioMarkers (ISOBM).
PY - 2015/7/29
Y1 - 2015/7/29
N2 - High-risk human papillomavirus (hrHPV) is an essential cause of cervical carcinoma and is also strongly related to anal cancer development. The hrHPV E6 oncoprotein plays a major role in carcinogenesis. We aimed to evaluate the frequency of hrHPV DNA and E6 oncoprotein in the anuses of women with cervical carcinoma. We analyzed 117 women with cervical cancer and 103 controls for hrHPV and the E6 oncogene. Positive test results for a cervical carcinoma included 66.7 % with hrHPV-16 and 7.7 % with hrHPV-18. One case tested positive for both HPV variants (0.9 %). The samples from the anal canal were positive for HPV-16 in 59.8 % of the cases. Simultaneous presence of HPV in the cervix and anal canal was found in 53.8 % of the cases. Regarding expression of E6 RNA, positivity for HPV-16 in the anal canal was found in 21.2 % of the cases, positivity for HPV-16 in the cervix was found in 75.0 %, and positivity for HPV-18 in the cervix was found in 1.9 %. E6 expression in both the cervix and anal canal was found in 19.2 % of the cases. In the controls, 1 % tested positive for HPV-16 and 0 % for HPV-18. Anal samples from the controls showed a hrHPV frequency of 4.9 % (only HPV16). The presence of hrHPV in the anal canal of women with cervical cancer was detected at a high frequency. We also detected E6 RNA expression in the anal canal of women with cervical cancer, suggesting that these women are at risk for anal hrHPV infection.
AB - High-risk human papillomavirus (hrHPV) is an essential cause of cervical carcinoma and is also strongly related to anal cancer development. The hrHPV E6 oncoprotein plays a major role in carcinogenesis. We aimed to evaluate the frequency of hrHPV DNA and E6 oncoprotein in the anuses of women with cervical carcinoma. We analyzed 117 women with cervical cancer and 103 controls for hrHPV and the E6 oncogene. Positive test results for a cervical carcinoma included 66.7 % with hrHPV-16 and 7.7 % with hrHPV-18. One case tested positive for both HPV variants (0.9 %). The samples from the anal canal were positive for HPV-16 in 59.8 % of the cases. Simultaneous presence of HPV in the cervix and anal canal was found in 53.8 % of the cases. Regarding expression of E6 RNA, positivity for HPV-16 in the anal canal was found in 21.2 % of the cases, positivity for HPV-16 in the cervix was found in 75.0 %, and positivity for HPV-18 in the cervix was found in 1.9 %. E6 expression in both the cervix and anal canal was found in 19.2 % of the cases. In the controls, 1 % tested positive for HPV-16 and 0 % for HPV-18. Anal samples from the controls showed a hrHPV frequency of 4.9 % (only HPV16). The presence of hrHPV in the anal canal of women with cervical cancer was detected at a high frequency. We also detected E6 RNA expression in the anal canal of women with cervical cancer, suggesting that these women are at risk for anal hrHPV infection.
KW - Anus
KW - Cervical cancer
KW - HPV
KW - HPV DNA
KW - HPV E6
KW - HPV RNA
UR - http://www.scopus.com/inward/record.url?scp=84938207512&partnerID=8YFLogxK
U2 - 10.1007/s13277-015-3205-9
DO - 10.1007/s13277-015-3205-9
M3 - Article
C2 - 25677903
AN - SCOPUS:84938207512
SN - 1010-4283
VL - 36
SP - 5399
EP - 5405
JO - Tumor Biology
JF - Tumor Biology
IS - 7
ER -