Clinical characteristics of women diagnosed with carcinoma who tested positive for cervical and anal high-risk human papillomavirus DNA and E6 RNA

Carlos A.R. Veo, Sarhan S. Saad, José Humberto T.G. Fregnani, Cristovam Scapulatempo-Neto, Audrey Tieko Tsunoda, Júlio César Possati Resende, Adriana Tarlá Lorenzi, Allini Mafra, Claudia Cinti, Ismael Dale Cotrim, Luciana Albina Reis Rosa, Cristina Mendes de Oliveira, Toni Ricardo Martins, Cristiane Centrone, José Eduardo Levi, Adhemar Longatto-Filho*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

High-risk human papillomavirus (hrHPV) is an essential cause of cervical carcinoma and is also strongly related to anal cancer development. The hrHPV E6 oncoprotein plays a major role in carcinogenesis. We aimed to evaluate the frequency of hrHPV DNA and E6 oncoprotein in the anuses of women with cervical carcinoma. We analyzed 117 women with cervical cancer and 103 controls for hrHPV and the E6 oncogene. Positive test results for a cervical carcinoma included 66.7 % with hrHPV-16 and 7.7 % with hrHPV-18. One case tested positive for both HPV variants (0.9 %). The samples from the anal canal were positive for HPV-16 in 59.8 % of the cases. Simultaneous presence of HPV in the cervix and anal canal was found in 53.8 % of the cases. Regarding expression of E6 RNA, positivity for HPV-16 in the anal canal was found in 21.2 % of the cases, positivity for HPV-16 in the cervix was found in 75.0 %, and positivity for HPV-18 in the cervix was found in 1.9 %. E6 expression in both the cervix and anal canal was found in 19.2 % of the cases. In the controls, 1 % tested positive for HPV-16 and 0 % for HPV-18. Anal samples from the controls showed a hrHPV frequency of 4.9 % (only HPV16). The presence of hrHPV in the anal canal of women with cervical cancer was detected at a high frequency. We also detected E6 RNA expression in the anal canal of women with cervical cancer, suggesting that these women are at risk for anal hrHPV infection.

Original languageEnglish
Pages (from-to)5399-5405
Number of pages7
JournalTumor Biology
Volume36
Issue number7
DOIs
Publication statusPublished - 29 Jul 2015
Externally publishedYes

Keywords

  • Anus
  • Cervical cancer
  • HPV
  • HPV DNA
  • HPV E6
  • HPV RNA

Fingerprint

Dive into the research topics of 'Clinical characteristics of women diagnosed with carcinoma who tested positive for cervical and anal high-risk human papillomavirus DNA and E6 RNA'. Together they form a unique fingerprint.

Cite this