TY - JOUR
T1 - Circulating leucocyte and lymphocyte subpopulations before and after intensive endurance exercise to exhaustion
AU - Gabriel, Holger
AU - Urhausen, Axel
AU - Kindermann, Wilfried
PY - 1991/11
Y1 - 1991/11
N2 - Seventeen healthy cyclists [age 20.8 (SD 4.8) years; body mass 68.3 (SD 7.7) kg; body fat, 11.4 (SD 2.6) %; height, 179.1 (SD 5.9) cm; {Mathematical expression} 60.9 (SD 7.4) ml · kg-1 · min-1] conducted intensive endurance exercise to exhaustion (stress test, ST) on a cycle ergometer at 110% of their individual anaerobic threshold [Than,individua; exercise intensity, 3.97 (SD 0.6) W · kg-1 ; duration, 23.9 (SD 8.3) min; maximal lactate concentration, 7.39 (SD 2.59) mmol · 1-1]. The distribution of leucocyte subpopulations was measured flow cytometrically: before, immediately after (0), 5 (+5), 30 (+30) and 60 (+60) min after ST. The lymphocytes (0 min) and granulocytes (+60 min) were mainly responsible for the increase of leucocytes. Lymphocytes were significantly lower at +30 and + 60 min than before. CD3-CD16/CD56+ (+480%) and CD8+-lymphocytes (+211%) increased at 0 min more than the other lymphocyte subpopulations (CD3+-cells, +100%; CD4+ cells, +56%; CD19+-cells, +64%). CD3-CD16/CD56+-and CD8+-cells also were mainly responsible for the decreased values of lymphocytes at +30 min and +60 min compared to before. At 0 min naive CD8+ cells (CD45RA+, CD45RO-) increased more than memory CD8+-cells (CD45RA-, CD45RO+). Changes of naive and memory CD4+-cells did not differ. All lymphocyte subpopulations, in particular CD8+- and CD3-CD16/CD56+-cells, decreased rapidly between 0 min and 5 min. We conclude that an intensive endurance exercise to exhaustion causes a mobilisation of lymphocytes, especially of natural killer cells (CD3-CD16/CD56+) and naive, unprimed CD8+ cells (CD45RA+, CD45RO-) which may be transported to injured muscles. The decreased cell numbers of the latter subpopulations are possibly one reason for the susceptibility to infections during the first hours after exercise. Furthermore, an exact definition of the intensity of exercise and times of taking blood is essential for comparing results describing cell parameters during or after exercise.
AB - Seventeen healthy cyclists [age 20.8 (SD 4.8) years; body mass 68.3 (SD 7.7) kg; body fat, 11.4 (SD 2.6) %; height, 179.1 (SD 5.9) cm; {Mathematical expression} 60.9 (SD 7.4) ml · kg-1 · min-1] conducted intensive endurance exercise to exhaustion (stress test, ST) on a cycle ergometer at 110% of their individual anaerobic threshold [Than,individua; exercise intensity, 3.97 (SD 0.6) W · kg-1 ; duration, 23.9 (SD 8.3) min; maximal lactate concentration, 7.39 (SD 2.59) mmol · 1-1]. The distribution of leucocyte subpopulations was measured flow cytometrically: before, immediately after (0), 5 (+5), 30 (+30) and 60 (+60) min after ST. The lymphocytes (0 min) and granulocytes (+60 min) were mainly responsible for the increase of leucocytes. Lymphocytes were significantly lower at +30 and + 60 min than before. CD3-CD16/CD56+ (+480%) and CD8+-lymphocytes (+211%) increased at 0 min more than the other lymphocyte subpopulations (CD3+-cells, +100%; CD4+ cells, +56%; CD19+-cells, +64%). CD3-CD16/CD56+-and CD8+-cells also were mainly responsible for the decreased values of lymphocytes at +30 min and +60 min compared to before. At 0 min naive CD8+ cells (CD45RA+, CD45RO-) increased more than memory CD8+-cells (CD45RA-, CD45RO+). Changes of naive and memory CD4+-cells did not differ. All lymphocyte subpopulations, in particular CD8+- and CD3-CD16/CD56+-cells, decreased rapidly between 0 min and 5 min. We conclude that an intensive endurance exercise to exhaustion causes a mobilisation of lymphocytes, especially of natural killer cells (CD3-CD16/CD56+) and naive, unprimed CD8+ cells (CD45RA+, CD45RO-) which may be transported to injured muscles. The decreased cell numbers of the latter subpopulations are possibly one reason for the susceptibility to infections during the first hours after exercise. Furthermore, an exact definition of the intensity of exercise and times of taking blood is essential for comparing results describing cell parameters during or after exercise.
KW - Exercise
KW - Leucocytes
KW - Lymphocyte subpopulations
KW - Memory cells
KW - Natural killer cells
UR - http://www.scopus.com/inward/record.url?scp=0025933480&partnerID=8YFLogxK
U2 - 10.1007/BF00868077
DO - 10.1007/BF00868077
M3 - Article
C2 - 1837265
AN - SCOPUS:0025933480
SN - 0301-5548
VL - 63
SP - 449
EP - 457
JO - European Journal of Applied Physiology and Occupational Physiology
JF - European Journal of Applied Physiology and Occupational Physiology
IS - 6
ER -