TY - JOUR
T1 - Chemically contaminated eel fed to pregnant and lactating mouse dams causes hyperactivity in their offspring
AU - Dridi, Imen
AU - Soualeh, Nidhal
AU - Bohn, Torsten
AU - Soulimani, Rachid
AU - Bouayed, Jaouad
N1 - Funding Information:
This work was supported by the Agence Nationale de la Recherche (ANR-11-CESA-000), and the European Regional Development Fund (ERDF). River eels used in this study were fished for a scientific study sponsored by the Service Public de Wallonie, DGO 'Agriculture, Ressources Naturelles et Environnement, n° 10/65/593'. The authors thank Prof. P. Poncin for river eel fishing, and Prof. J-P. Thomé, Prof. G. Eppe, and Dr. G. Scholl from the CART of the ULg for their assistance with the analytical behavior
Publisher Copyright:
© 2017 Hogrefe.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - This study examined whether perinatal exposure to polluted eels (Anguilla anguilla L.) induces changes in the locomotor activity of offspring mice across lifespan (post-natal days (PNDs) 47 - 329), using the open field and the home cage activity tests. Dams were exposed during gestation and lactation, through diets enriched in eels naturally contaminated with pollutants including PCBs. Analysis of the eel muscle focused on the six non-dioxin-like (NDL) indicator PCBs (Σ6 NDL-PCBs: 28, 52, 101, 138, 153 and 180). Four groups of dams (n = 10 per group) received either a standard diet without eels or eels (0.8 mg/kg/day) containing 85, 216, or 400 ng/kg/day of Σ6 NDL-PCBs. The open field test showed that early-life exposure to polluted eels increased locomotion in female offspring of exposed dams but not in males, compared to controls. This hyperlocomotion appeared later in life, at PNDs 195 and 329 (up to 32 % increase, p < 0.05). In addition, overactivity was observed in the home cage test at PND 305: exposed offspring females showed a faster overall locomotion speed (3.6 - 4.2 cm/s) than controls (2.9 cm/s, p < 0.05); again, males remained unaffected. Covered distances in the home cage test were only elevated significantly in offspring females exposed to highest PCB concentrations (3411 ± 590 cm vs. 1377 ± 114 cm, p < 0.001). These results suggest that early-life exposure to polluted eels containing dietary contaminants including PCBs caused late, persistent and gender-dependent neurobehavioral hyperactive effects in offspring mice. Furthermore, female hyperactivity was associated with a significant inhibition of acetylcholinesterase activity in the hippocampus and the prefrontal cortex.
AB - This study examined whether perinatal exposure to polluted eels (Anguilla anguilla L.) induces changes in the locomotor activity of offspring mice across lifespan (post-natal days (PNDs) 47 - 329), using the open field and the home cage activity tests. Dams were exposed during gestation and lactation, through diets enriched in eels naturally contaminated with pollutants including PCBs. Analysis of the eel muscle focused on the six non-dioxin-like (NDL) indicator PCBs (Σ6 NDL-PCBs: 28, 52, 101, 138, 153 and 180). Four groups of dams (n = 10 per group) received either a standard diet without eels or eels (0.8 mg/kg/day) containing 85, 216, or 400 ng/kg/day of Σ6 NDL-PCBs. The open field test showed that early-life exposure to polluted eels increased locomotion in female offspring of exposed dams but not in males, compared to controls. This hyperlocomotion appeared later in life, at PNDs 195 and 329 (up to 32 % increase, p < 0.05). In addition, overactivity was observed in the home cage test at PND 305: exposed offspring females showed a faster overall locomotion speed (3.6 - 4.2 cm/s) than controls (2.9 cm/s, p < 0.05); again, males remained unaffected. Covered distances in the home cage test were only elevated significantly in offspring females exposed to highest PCB concentrations (3411 ± 590 cm vs. 1377 ± 114 cm, p < 0.001). These results suggest that early-life exposure to polluted eels containing dietary contaminants including PCBs caused late, persistent and gender-dependent neurobehavioral hyperactive effects in offspring mice. Furthermore, female hyperactivity was associated with a significant inhibition of acetylcholinesterase activity in the hippocampus and the prefrontal cortex.
KW - Acetylcholinesterase activity
KW - Eels
KW - Hyperactivity
KW - Late effects
KW - Offspring mice
KW - PCBs
KW - Perinatal exposure
UR - http://www.scopus.com/inward/record.url?scp=85019341315&partnerID=8YFLogxK
U2 - 10.1024/0300-9831/a000288
DO - 10.1024/0300-9831/a000288
M3 - Article
AN - SCOPUS:85019341315
SN - 0300-9831
VL - 86
SP - 36
EP - 47
JO - International Journal for Vitamin and Nutrition Research
JF - International Journal for Vitamin and Nutrition Research
IS - 1-2
ER -